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目的:比较研究松杉灵芝水溶性多糖(Fl_0-b)及其甲酸修饰产物(FI_0-b-H)对人炎症性细胞因子产生的影响.方法:用放射免疫分析法(RIA)及逆转录聚合酶链反应(RT-PCR)分析法研究FI_0-b和Fl_0-b-H对人组织瘤细胞(THP-1)和人外周血单核细胞(PBMC)分泌的各种与炎症有关的细胞因子(白介素-1 α,6,8,肿瘤坏死因子α)及对白介素-1α,肿瘤坏死因子αmRNA表达的影响.结果:FI_0-b和FI_0-b-H(浓度分别为4,40,400 mg/L)显著抑制LPS 10 mg/L协同PMA 200 nmol/L诱导的THP-1细胞和PBMC细胞产生IL-1α和TNF α的量.当低浓度刺激剂(LPS 1mg/L协同PMA 200 nmol/L)刺激THP-1细胞时,FI_0-b-H明显提高白介素-8的产生;但当高浓度刺激剂(LPS 10 mg/L协同200 nmol/L PMA)刺激THP-1-细胞时,FI_0-b-H却明显抑制白介素-8的产生.FI_0-b-和FI_0-b-H对THP-1细胞白介素-1α和肿瘤坏死因子α的mRNA表达有显著作用.结论:与FI_0-b相比,FI_0-b-H在人炎症性细胞因子的产生方面表现出较强的活性.松杉灵芝菌丝体水溶性多糖在不同的刺激条件下,具有双向免疫调节作用,并具有一定的剂量依赖关系.
OBJECTIVE: To compare the effects of Fl_0-b and its formic acid-modified product (FI_0-bH) on the production of human inflammatory cytokines. Methods: Radioimmunoassay (RIA) and reverse transcription polymerase The chain reaction (RT-PCR) assay was used to investigate the various cytokines associated with inflammation (human interleukin-1) secreted by FI_0-b and Fl_0-bH on human tissue tumor cells (THP-1) and human peripheral blood mononuclear cells (PBMC). 1 α,6,8, Tumor necrosis factor α and its effects on interleukin-1α and tumor necrosis factor α mRNA expression. Results: FI_0-b and FI_0-bH (concentrations 4, 40, 400 mg/L, respectively) significantly inhibited LPS The amount of IL-1α and TNFα produced by THP-1 cells and PBMCs induced by 10 mg/L PMA 200 nmol/L was stimulated by low concentration of stimulant (LPS 1 mg/L in combination with PMA 200 nmol/L). FI_0-bH significantly increased interleukin-8 production in cells, but FI_0-bH significantly inhibited interleukin-8 when THP-1-cells were stimulated with a high concentration of stimulant (LPS 10 mg/L in combination with 200 nmol/L PMA). The production of FI_0-b- and FI_0-bH had significant effects on the mRNA expression of interleukin-1α and tumor necrosis factor α in THP-1 cells. Conclusion: Compared with FI_0-b, FI_0-bH in human inflammatory cytokines Production It showed strong activity in the aspect of life. The water-soluble polysaccharide of mycelia of ganoderma lucidum had two-way immunomodulatory effect under different stimulating conditions and had a certain dose-dependent relationship.