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为分析转化生长因子 ( TGF- α)在肺纤维化过程中的作用 ,本实验选用转基因小鼠与野生型小鼠来研究肺损伤后两者纤维化的差异。转基因小鼠的 TGF- α基因第 3外显子经插入重组失活而不能表达 TGF-α蛋白。动物经 0 .0 75U博莱霉素一次性气管滴注建立肺损伤模型 ,并以生理盐水作
In order to analyze the role of transforming growth factor (TGF-α) in pulmonary fibrosis, transgenic mice and wild-type mice were selected to study the differences in fibrosis between the two groups after lung injury. Transgenic mice TGF-α gene exon 3 by insert recombinant inactivation can not express TGF-α protein. The animal model of lung injury was established by intraperitoneal instillation of 0 75 U bleomycin with saline