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本研究从陆地棉TM-1基因组中鉴定出72个XTH家族基因,编码木葡聚糖内转糖苷酶/水解酶(XTH,xyloglucan endotransglycosylase/hydrolase),分别命名为Gh XTH01~Gh XTH72,分析了其基因结构、保守基序、系统进化、理化性质、亚细胞定位,并探究其在棉纤维发育不同时期的表达规律。结果表明,XTH家族基因分布在除At 07、Dt 07以外的24条棉花染色体上,根据系统发育树,将XTH家族基因分为3个亚组;XTH氨基酸序列有3个保守基序,保守性较强;多数XTH蛋白定位在细胞外。根据XTHs在纤维发育不同时期的表达量变化,将其分为4类。通过构建陆地棉与拟南芥XTH氨基酸序列进化树,推测Gh XTH15、Gh XTH28、Gh XTH36、Gh XTH49、Gh XTH59、Gh XTH62、Gh XTH63等基因在棉纤维发育过程中发挥重要作用。通过比较XTH家族基因在不同纤维品质陆地棉品种中的表达差异,推测在优质棉花品种中优势表达基因Gh XTH03、Gh XTH12、Gh XTH17、Gh XTH22、Gh XTH23、Gh XTH28、Gh XTH33、Gh XTH44、Gh XTH46、Gh XTH59等在纤维发育伸长过程中可能发挥着重要作用。上述结果为研究陆地棉XTH基因家族在棉纤维发育中的功能提供了参考依据。
In this study, 72 XTH family genes were identified from G. tumefaciens TM-1 genome and encoded xyloglucan endotransglycosylase / hydrolase (XTH), named as Gh XTH01 ~ Gh XTH72 respectively Its gene structure, conserved motifs, phylogenetic, physicochemical properties and subcellular localization were studied. The expression patterns of these genes in different stages of cotton fiber development were also explored. The results showed that XTH family genes were distributed on 24 cotton chromosomes except At 07 and Dt 07, and the XTH family genes were divided into three subgroups according to the phylogenetic tree. The XTH amino acid sequence had 3 conserved motifs Strong; Most XTH protein localization in the extracellular. According to the expression of XTHs at different stages of fiber development, they were divided into four groups. By constructing the phylogenetic tree of the amino acid sequence of XTH from Upland cotton and Arabidopsis thaliana, it is speculated that Gh XTH15, Gh XTH28, Gh XTH36, Gh XTH49, Gh XTH59, Gh XTH62 and Gh XTH63 play an important role in cotton fiber development. By comparing the expression differences of XTH family genes in Upland cotton cultivars with different fiber quality, we speculated that the dominant genes of Gh XTH03, Gh XTH12, Gh XTH17, Gh XTH22, Gh XTH23, Gh XTH28, Gh XTH33, Gh XTH44, Gh XTH46, Gh XTH59, etc. may play an important role in the process of fiber elongation. These results provide a reference for studying the function of XTH gene family in cotton fiber development.