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建立了测定蛛网膜下腔出血患者（ＳＡＨ）脑脊液中血小板活化因子（ＰＡＦ）质量浓度的高效薄层扫描方法。薄层板为高效硅胶Ｇ板（１００ ｍ ｍ ×１００ ｍ ｍ ），展开剂为Ｖ（氯仿）ｖＶ（甲醇）ｖＶ（水）＝ ６５ｖ３５ｖ６，下层液展开，显色剂为１００ ｇ／Ｌ磷钼酸溶液，展开距９０ ｍ ｍ ，扫描波长６３０ ｎｍ 。方法的线性范围０．５ ～２．５ μｇ／Ｌ，相关系数０．９９９ ０，最小检测质量浓度５０ ｎｇ／Ｌ，平均回收率９８．６％ 。运用所建立的方法测定了１６例ＳＡＨ患者发病后脑脊液和１０例非神经系统疾病患者脑脊液中ＰＡＦ的质量浓度及其变化规律，为临床提供了诊断依据。
A high-performance thin-layer scanning method for the determination of platelet-activating factor (PAF) in cerebrospinal fluid of patients with subarachnoid hemorrhage (SAH) was established. The TLC plate was a high efficiency silica gel G plate (100 m × 100 m m). The developing agent was V (chloroform) vV (methanol) vV (water) = 65 v35v6. Acid solution, developed 90 m m away, scanning wavelength 630 nm. The linear range of the method was 0.5-2.5 μg / L with a correlation coefficient of 0.999 0. The minimum detectable concentration was 50 ng / L and the average recovery was 98.6%. The established method was used to determine the concentration and variation of PAF in cerebrospinal fluid of 16 patients with SAH after onset and 10 patients with non-neurological diseases, which provided the basis for clinical diagnosis.