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目的:通过使用慢病毒技术,建立肺转移裸鼠模型,观察miRNA-194对于骨肉瘤增殖和转移的影响,为研究miRNA-194在骨肉瘤中的作用及进一步的治疗提供理论依据。方法:使用慢病毒技术对骨肉瘤细胞系U2-OS进行转染后分组,然后将转染后的各组细胞注入裸鼠体内建立肺转移模型。5周后将裸鼠处死,观察和比较原位及肺部肿瘤的大小。结果:1)mi RNA-194下调组裸鼠的原位肿瘤的体积(3920±860 mm~3)和重量(2.15±0.32 g)明显的大于其余各组(P<0.05);2)miRNA-194上调组的肺部情况(36.7±12.4个)明显的优于其余各组(P<0.05);3)病理学检测证实其确实为原位骨肉瘤及肺部转移病灶。结论:miR-194可以明显的在裸鼠体内抑制骨肉瘤的增殖及肺部的转移,从而显示mi R-194在动物水平在骨肉瘤中呈现明显的抑癌基因的作用,为接下来关于miRNA-194的各项实验奠定了良好的实验基础。
Objective: To study the effect of miRNA-194 on the proliferation and metastasis of osteosarcoma by using lentivirus technology to establish a model of lung metastasis in nude mice and to provide a theoretical basis for studying the role of miRNA-194 in osteosarcoma and further treatment. METHODS: The osteosarcoma cell line U2-OS was transfected with lentivirus and then sub-divided into groups. Then the transfected cells were injected into nude mice to establish lung metastasis model. After 5 weeks, the nude mice were sacrificed and the size of the tumors in situ and in the lung was observed and compared. Results: (1) The volume of tumor in situ (3920 ± 860 mm ~ 3) and weight (2.15 ± 0.32 g) of mi RNA-194 down-regulated group were significantly greater than those of other groups (P <0.05) 194 lung tissue (36.7 ± 12.4) in the up-regulated group was significantly better than the other groups (P <0.05); 3) pathological examination confirmed that it was indeed in situ osteosarcoma and lung metastasis. Conclusion: miR-194 can significantly inhibit the proliferation and lung metastasis of osteosarcoma in nude mice, which shows that mi R-194 shows a significant tumor suppressor gene in osteosarcoma at animal level. -194 experiments have laid a good foundation for the experiment.