论文部分内容阅读
目的:探讨妊娠期糖尿病(GDM)患者胎盘中肝细胞生长因子(HGF)、晚期糖基化终产物受体(RAGE)的表达情况及其临床意义。方法:选择2011年1月～2012年12月该院各项资料完整的45例晚孕GDM孕妇作为观察组,另选择同期来院产前检查(产检)并分娩的正常晚孕孕妇43例作为对照组。采用免疫组织化学法检测两组患者胎盘中HGF、RAGE的表达水平及表达位点,并采用RT-PCR法测定HGF mRNA和RAGE mRNA水平。结果:观察组胎盘中HGF mRNA及表达水平明显低于对照组,差异有统计学意义(P<0.01),而RAGE mRNA及表达水平明显高于对照组,差异有统计学意义(P<0.01)。结论:GDM患者胎盘中HGF、RAGE的表达情况与正常妊娠孕妇有明显的差异,提示了两者对GDM的诊断和治疗有着重要的临床意义。
Objective: To investigate the expression of hepatocyte growth factor (HGF) and advanced glycation end products receptor (RAGE) in placenta of patients with gestational diabetes mellitus (GDM) and its clinical significance. Methods: Forty-five pregnant women with GDM in the hospital from January 2011 to December 2012 were enrolled as observation group. Forty-three pregnant women of the same period were enrolled in this study. group. Immunohistochemistry was used to detect the expression and expression of HGF and RAGE in placentas of both groups. The levels of HGF mRNA and RAGE mRNA were determined by RT-PCR. Results: The expression of HGF mRNA in the placenta of the observation group was significantly lower than that of the control group (P <0.01), while the expression of RAGE mRNA and protein in the placenta of the observation group was significantly higher than that in the control group (P <0.01) . Conclusion: The expression of HGF and RAGE in placenta of GDM patients is significantly different from that of normal pregnant women, suggesting that both of them have important clinical significance in the diagnosis and treatment of GDM.