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目的:观察橄榄油对去势后大鼠骨代谢和骨密度的影响,探讨并分析橄榄油防治绝经后骨质疏松症(PMOP)的有效性及机理。方法:将30只5～6个月龄清洁型SD雌性大鼠进行随机分成四组:①假手术组(Sham组)、②去卵巢组(OVX组)、③去卵巢+橄榄油组(OVX+Olive)、④去卵巢+雌激素组(OVX+E)。治疗组用药:(OVX+Olive)组及(OVX+E)组均采用经口灌胃方式进行用药,按1 ml/100 g体重,1次/天灌胃,连续12周。12周后分别左心室取血,检测血中雌二醇(E2)、血钙、血碱性磷酸酶(ALP)、白介素-6(IL-6)水平,放血处死后取出腰椎及左侧股骨行双能X线骨密度(DEXA)测定。结果:OVX组中血E2明显低于Sham组和治疗组,差异有统计学意义(P<0.01、P<0.05),ALP、IL-6值显著高于其余三组,差异有统计学意义(P<0.05);治疗组中E2、ALP、IL-6与Sham组差异无统计学意义(P>0.05);四组中血钙值差异均无统计学意义(P>0.05)。去势橄榄油组与雌激素组骨密度平均值均较OVX组高,差异有统计学意义(P<0.05),但该两组间差异无统计学意义(P>0.05)。结论:橄榄油能有效地减轻大鼠卵巢切除术引起的骨质丢失,可能通过补充植物雌激素发挥拟雌激素样作用。
Objective: To observe the effects of olive oil on bone metabolism and bone mineral density (BMD) after castration in ovariectomized rats, and to explore and analyze the effectiveness and mechanism of olive oil in preventing and treating postmenopausal osteoporosis (PMOP). Methods: Thirty SD female SD rats aged 5-6 months were randomly divided into four groups: ① Sham group, ② ovariectomized group (OVX group), ③ ovariectomized + olive oil group (OVX + Olive), ovariectomized + estrogen group (OVX + E). The treatment group: (OVX + Olive) group and (OVX + E) group were administered by oral gavage, by 1 ml / 100 g body weight, 1 / day gavage for 12 weeks. Twelve weeks later, the left ventricular blood was taken for determination of the level of estradiol (E2), serum calcium, ALP and IL-6 in the blood and the lumbar spine and left femur Line dual-energy X-ray absorptiometry (DEXA) determination. Results: The serum level of E2 in OVX group was significantly lower than that in Sham group and treatment group (P <0.01, P <0.05), ALP and IL-6 levels were significantly higher than those in the other three groups P <0.05). There was no significant difference in E2, ALP, IL-6 and Sham between the treatment group and the Sham group (P> 0.05). There was no significant difference in the serum calcium between the four groups (P> 0.05). The mean bone mineral density in castrated olive oil group and estrogen group was higher than that in OVX group (P <0.05), but there was no significant difference between the two groups (P> 0.05). Conclusion: Olive oil can effectively reduce the bone loss caused by ovariectomy in rats, and may play a role in the estrogen-like effect by supplementing phytoestrogens.