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Aim:To investigate the inhibitory effect of a new compound of GLB on tumormetastasis in vivo and analyze its actions on tumor cell adhesion to clarify itsmechanism.Methods:The effect of GLB on tumor metastasis was analyzed byLewis lung carcinoma model.The pathological morphology of lung alveolar wasevaluated by hematoxylin-eosin staining.The effect of GLB on the proliferation ofhuman prostate cancer cell(PC-3M,with a high metastatic characteristic)wasstudied using the MTT method,and its actions on PC-3M cell adhesion to humanumbilical vein endothelial cells(HUVEC)and laminin were analyzed in vitro.Results:GLB(100 mg·kg~(-1)·d~(-1)for 28 d,ig)reduced the number of lung colonies ofLewis lung carcinoma metastasis significantly(P<0.05).Simultaneously,GLBcould mitigate the damage of lung alveolar caused by metastasic tumor deposits.In vitro,GLB inhibited dramatically the adhesion of PC-3M cells to HUVEC(P<0.01)and laminin(P<0.05),without cytotoxic or anti-proliferative action on PC-3Mcells.Conclusion:GLB has anti-tumor metastatic activity,which partly dependson its inhibition of tumor adhesion.
Aim: To investigate the inhibitory effect of a new compound of GLB on tumor metastasis in vivo and analyze its actions on tumor cell adhesion to clarify its mechanism. Methods: The effect of GLB on tumor metastasis was analyzed by Lewis lung carcinoma model. Pathological morphology of lung alveolar wasevaluated by hematoxylin-eosin staining. The effect of GLB on the proliferation of human prostate cancer cell (PC-3M, with a high metastatic characteristic) was studied using the MTT method, and its actions on PC-3M cell adhesion to humanumbilical vein endothelial cells (HUVEC) and laminin were analyzed in vitro. Results: GLB (100 mg · kg -1 · d -1 for 28 d, ig) reduced the number of lung colonies of Lewis lung carcinoma metastasis significantly (P < 0.05). Simultaneously, GLBcould mitigate the damage of lung alveolar caused by metastasic tumor deposits. In vitro, GLB inhibitors dramatically the adhesion of PC-3M cells to HUVEC (P <0.01) and laminin -proliferative action on PC-3Mcell s.Conclusion: GLB has anti-tumor metastatic activity, which partly dependson its inhibition of tumor adhesion.