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支气管哮喘发病机理未完全明确,近年发现GCR功能及T淋巴细胞免疫缺陷与本病有关。本文报导20例哮喘患儿静脉血淋巴细胞GCR、T淋巴细胞亚群及Ig的测定结果,试图探讨其在哮喘发病中的作用。材料与方法本组20例均为本科住院患儿,男14例,女6例,年龄5个月至10岁.均为哮喘发作期,1周内未使用激素者.淋巴细胞来源于哮喘患儿静脉血.试剂及方法 ①静脉血GCR测定:以氘记地塞米松(~3H—Dex)作为配体,采用一点分析法测定完整淋巴细胞内的GCR.~3H—Dex为英国Amersham产品。②T淋巴细胞亚群检测:采用微量细胞毒法测定T淋巴细胞亚群CD_3、CD_4和CD_5,并计算CD_4/CD_8比值。
The pathogenesis of bronchial asthma is not completely clear, in recent years found GCR function and T lymphocyte immune deficiency and the disease. This article reports the results of determination of GCR, T lymphocyte subsets and Ig in venous blood lymphocytes in 20 asthmatic children and attempts to explore its role in the pathogenesis of asthma. Materials and Methods The group of 20 patients were hospitalized infants, 14 males and 6 females, aged 5 months to 10 years of age are asthma attack period, one week without the use of hormones. Lymphocytes from asthma Pediatric venous blood Reagents and methods ① Venous blood GCR determination: dexamethasone deuterium (~ 3H-Dex) was used as a ligand and the intracytoplasmic GCR was determined by one-point analysis. ~ 3H-Dex was produced by Amersham, UK. ②T lymphocyte subsets detection: T lymphocyte subsets CD_3, CD_4 and CD_5 were determined by the method of micro-cytotoxicity, and the ratio of CD_4 / CD_8 was calculated.