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目的本文检测CEAmRNA、CEA在结直肠癌、结肠息肉、正常人群中的表达,并探讨其在结直肠癌诊断与治疗中的意义。方法选取结直肠癌患者60例,结肠息肉患者40例,正常对照40例,分别用逆转录-PCR(RT-PCR)法检测其外周血中CEAmRNA的表达,酶联免疫吸附试验(ELISA)测定其血清CEA蛋白的含量。结果60例结直肠癌患者CEAmRNA阳性率为68·3%,健康对照组有1例呈弱阳性,两者比较有显著性差异(P<0.001);40例结肠息肉患者CEAmRNA仅3例阳性。CEAmRNA阳性表达率在DukesC+D期患者明显高于DukesA+B期。外周血CEAmRNA的表达与其它病理因素无明显关系。CEAmRNA阳性表达与肝转移密切相关。结论外周血细胞中CEAmRNA的表达与肿瘤分期及肝转移相关,有可能作为结直肠癌转移的一个辅助监测指标。对结肠息肉患者进行外周血CEAmRNA检测,有助于发现癌前病变。
Objective To detect the expression of CEAmRNA and CEA in colorectal cancer, colonic polyps and normal population and to explore its significance in the diagnosis and treatment of colorectal cancer. Methods Sixty patients with colorectal cancer, 40 patients with colonic polyps and 40 normal controls were enrolled in this study. CEA mRNA expression in peripheral blood was detected by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) The serum CEA protein content. Results The positive rate of CEA mRNA in 60 patients with colorectal cancer was 68.3%. One patient in the healthy control group was weakly positive (P <0.001). Only 3 patients had CEAmRNA in 40 patients with colonic polyps. The positive rate of CEA mRNA in DukesC + D patients was significantly higher than that in DukesA + B patients. The expression of CEAmRNA in peripheral blood had no significant relationship with other pathological factors. CEA mRNA positive expression is closely related to liver metastasis. Conclusion The expression of CEAmRNA in peripheral blood cells correlates with tumor stage and liver metastasis, which may be used as a secondary monitoring indicator of metastasis of colorectal cancer. Peripheral blood CEAmRNA detection in patients with colon polyps can help detect precancerous lesions.