【摘 要】
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Robinia pseudoacacia is an important afforesta-tion tree introduced to China in 1878. In the present study, we examined the genetic diversity among 687 strains rep-resenting four improved varieties and two secondary prov-enances, comprising 641 clones and
【机 构】
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Beijing Advanced Innovation Center for Tree Breeding By Molecular Design,Engineering Technology Rese
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Robinia pseudoacacia is an important afforesta-tion tree introduced to China in 1878. In the present study, we examined the genetic diversity among 687 strains rep-resenting four improved varieties and two secondary prov-enances, comprising 641 clones and 46 seedlings. Ninety-one simple sequence repeats (SSRs) were selected through segregation analysis and polymorphism characterization, and all sampled individuals were genotyped using well-characterized SSR markers. After excluding loci with non-neutral equilibrium, missing locus data and null alleles, we used 36 primer pairs to assess the genetic diversity of these germplasm resources, revealing vast genetic differentiation among the samples, with an average of 8.352 alleles per locus and a mean Shannon′s index of 1.302. At the popula-tion level, the partitioning of variability was assessed using analysis of molecular variance, which revealed 93％ and 7％ variation within and among collection sites, respectively. Four clusters were detected using structure analysis, indi-cating a degree of genetic differentiation among the six populations. Insights into the genetic diversity and structure of R. pseudoacacia provide a theoretical basis for the con-servation, breeding and sustainable development in China.
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