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目的探讨NQO1基因C609T变异与大肠癌易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)基因分型技术,对268对大肠癌患者和对照者NQO1基因cDNA609位点多态性进行测定。结果病例组中基因型为C/C、C/T、T/T的分别有68,149,69例,分别占23.77%,52.10%和24.13%;对照组分别有116,126,44人,占40 56%,44.06%和15.38%;3种基因型频率在2组中分布差异均有统计学意义(P<0.05);以C/C基因型作为参照基因型,C/T和T/T2种基因型的调整OR分别为2.06(95%CI=1.37~3.10)和2.64(95%CI=1.57~4.44);NQO1C609T变异基因型与吸烟、饮用水及食物类型存在交互作用而增加个体患大肠癌的危险。结论携带NQO1C609T,变异基因型个体患大肠癌的风险增加,且该变异基因型与环境因素具有协同致癌作用。
Objective To investigate the relationship between the C609T mutation of NQO1 gene and the susceptibility of colorectal cancer. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyping was used to determine the polymorphism of NQO1 gene 268 loci in 268 colorectal cancer patients and controls. Results There were 68,149,69 C / T and T / T genotypes in the case group, accounting for 23.77%, 52.10% and 24.13%, respectively. The control group had 116, 126 and 44 persons respectively, accounting for 40.56% , 44.06% and 15.38%, respectively. The frequencies of three genotypes in the two groups were significantly different (P <0.05). The genotypes of C / T and T / T2 genotypes (95% CI = 1.37 ~ 3.10) and 2.64 (95% CI = 1.57 ~ 4.44) respectively. The interaction between NQO1C609T genotype and smoking, drinking water and food type increased the risk of colorectal cancer . Conclusion The risk of colorectal cancer in NQO1C609T variant genotype individuals is increased, and this variant genotype has synergistic carcinogenic effects with environmental factors.