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从体内和体外 2方面研究锌对红细胞膜 Na+ ,K+ - ATP酶和 Ca2 + ,Mg2 + - ATP酶活性的影响。健康雄性 Wistar大鼠分别喂饲低锌、常量锌和高锌饲料 (饲料锌含量分别为 2 .2、2 8、12 8mg/kg) ,2 5天后各组随机取 8只动物腹主动脉取血后处死 ,测定血清锌含量和红细胞膜 Na+ ,K+ - ATP酶和 Ca2 + ,Mg2 + - ATP酶活性 ;各组剩余 6只动物改变其锌摄入量后继续喂饲 7天后 ,观察锌对红细胞膜 2种 ATP酶活性影响的敏感性。体外实验用新鲜制备的人红细胞膜 ,在不同浓度锌 (0、5、10、5 0、10 0和 5 0 0 μmol/L)溶液中温浴 ,观察 2种 ATP酶活性的变化趋势。结果表明 ,锌对红细胞膜的 2种 ATP酶活性均有影响 ,锌浓度过低或过高可抑制酶活性。与 Na+ ,K+ - ATP酶相比 ,Ca2 + ,Mg2 + - ATP酶对缺锌更敏感且补锌后不易恢复 ,但其对高锌有较强的耐受性。本次研究结果提示锌对于维持红细胞膜 ATP酶的正常活性有重要意义。
Effects of Zinc on Activities of Na +, K + - ATPase and Ca2 +, Mg2 + - ATPase in Erythrocyte Membrane from in Vitro and in. Healthy male Wistar rats were fed low zinc, zinc and high zinc diets, respectively (feed zinc content were 2.2,28,128mg / kg), 25 days after taking a random group of eight animal abdominal aorta The blood was sacrificed and the level of serum zinc and the activity of Na +, K + - ATPase and Ca2 +, Mg2 + - ATPase of erythrocyte membrane were determined. After the remaining 6 animals in each group changed their zinc intake and continued to feed for 7 days, Sensitivity of Erythrocyte Membrane 2 ATPase Activity. In vitro experiments with freshly prepared human erythrocyte membrane in different concentrations of zinc (0,5,10,5 0,10 0 and 500 micromol / L) solution in a warm bath to observe the trend of two ATPase activity. The results showed that zinc affected both ATPase activities of erythrocyte membrane, while zinc concentration was too low or too high to inhibit enzyme activity. Compared with Na +, K + - ATPase, Ca2 + and Mg2 + - ATPase are more sensitive to zinc deficiency and not easy to recover after zinc supplementation, but they are highly tolerant to high zinc. The results of this study suggest that zinc is important for maintaining the normal activity of the erythrocyte membrane ATPase.