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目的 研究弓形虫抗原对免疫宿主的保护性。 方法 用 SDS- PAGE大胶板电泳对弓形虫抗原进行分离 ,选择 4组份特异抗原带 (P2 2～ 2 6、P30、P35、P6 7)洗脱获得抗原后 ,分别与福氏完全佐剂混匀免疫小鼠 (每组 10只 ) ,发现P30诱导的小鼠抗弓形虫 Ig G抗体出现最早 ,滴度最高 ,其次为 P35。以毒力减弱的 RH株弓形虫对免疫后的各组小鼠进行攻击。 结果 对照组、P2 2～ 2 6、P30、P35和 P6 7组小鼠的平均存活时间分别为 7.1、7.8、8.3、8.0和 7.3d。 结论P30对小鼠具有较强的保护力 (P<0 .0 5 ) ,次为 P35。二者可能成为弓形虫疫苗的候选抗原
Objective To study the protective effect of Toxoplasma gondii antigen on immune host. Methods The Toxoplasma gondii antigens were isolated by SDS-PAGE electrophoresis and the antigen was obtained after the elution of 4 specific antigen bands (P2 2 ~ 2 6, P30, P35, P6 7) Immunized mice (10 mice in each group) were immunized with the earliest and highest titer of anti-toxoplasma IgG antibodies against P30 in mice, followed by P35. Toxicity of RH Toxoplasma gondii were used to challenge each group of mice after immunization. Results In the control group, the mean survival time of mice in P2 2 ~ 2 6, P30, P35 and P6 7 groups was 7.1, 7.8, 8.3, 8.0 and 7.3 days, respectively. Conclusion P30 has strong protective effect on mice (P <0.05), followed by P35. Both may be candidate antigens for the toxoplasma vaccine