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目的和方法 :用Fura 2 /AM .荧光显示测定细胞内游离Ca2 + 浓度 ([Ca2 + ]i)的方法 ,我们研究了牛磺酸 (Tau)对血管紧张素Ⅱ (AngⅡ )引起的培养心肌细胞 [Ca2 + ]i变化的影响。结果 :在有Ca2 + 和无Ca2 + 的缓冲液中 ,AngⅡ (1 ,1 0 ,1 0 0 ,1 0 0 0nmol/L)能浓度依赖性地引起 [Ca2 + ]i升高。在含Ca2 + 的缓冲液中 ,Tau(1 0 ,2 0mol/L)可依浓度地抑制AngⅡ (1 0 0nmol/L)引起的 [Ca2 + ]i升高 ;但在无Ca2 + 的缓冲液中 ,牛磺酸无此作用。用ryanodine(Rya ,1 μmol/L)预先耗竭细胞内贮存的Ca2 + 后 ,AngⅡ (1 0 0nmol/L)仍能引起 [Ca2 + ]i进一步升高 :AngⅡ的这一作用能被Tau(2 0mmol/L)显著抑制。结论 :在培养的乳鼠心肌细胞 ,Tau能够通过抑制AngⅡ引起的Ca2 + 内流而拮抗AngⅡ升高 [Ca2 + ]i的作用。
PURPOSE AND METHODS: The effect of taurine (Tau) on angiotensin Ⅱ-induced cultured myocardium was studied by Fura 2 / AM. Fluorescence measurement of intracellular free Ca 2+ concentration ([Ca 2+] i) Effect of changes in [Ca2 +] i in cells. RESULTS: Ang II (1 1, 100, 100 nmol / L) caused a [Ca 2+] i increase in a concentration-dependent manner in Ca 2+ -free and Ca 2+ -free buffers. In Ca2 + -containing buffer, Tau (100, 20 mol / L) inhibited the increase of [Ca2 +] i induced by AngⅡ (100 nmol / L) in a concentration- In taurine no such effect. AngⅡ (100 nmol / L) still induced a further increase of [Ca2 +] i after pre-depletion of intracellular Ca2 + by ryanodine (Rya, 1 μmol / L) 0mmol / L) significantly inhibited. Conclusion: In cultured neonatal rat cardiomyocytes, Tau can antagonize the increase of [Ca2 +] i by inhibiting the influx of Ca2 + induced by Ang Ⅱ.