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目的：研究血管紧张素ＩＩ（ＡｎｇＩＩ）对心肌细胞蛋白质合成速率和肌球蛋白重链（ｍｙｏｓｉｎｈｅａｖｙｃｈａｉｎ，ＭＨＣ）基因表达的影响。方法：采用放射性同位素氚（３Ｈ）－亮氨酸参入量方法观察培养心肌细胞蛋白质合成速率。应用斑点杂交方法分析ＡｎｇＩ对心肌细胞α－ＭＨＣ和β－ＭＨＣ基因表达的作用。结果：在培养的心肌细胞中加入ＡｎｇＩ可明显增加心肌细胞３Ｈ－亮氨酸的参入量，并可诱导心肌细胞α－ＭＨＣ和β－ＭＨＣ基因表达迅速增加，在一定范围内，两者均呈量效关系。ＡｎｇＩ阻断剂沙拉新（ｓａｒ－ａｌａｓｉｎ）可阻断ＡｎｇＩＩ的上述作用。结论：ＡｎｇＩＩ可增加心肌细胞蛋白质合成速率，促进心肌细胞α－ＭＨＣ和β－ＭＨＣ基因表达。
Objective: To study the effect of angiotensin II (AngII) on the protein synthesis rate of myocytes and the expression of myosin heavy chain (MHC) genes. METHODS: The protein synthesis rate of cultured cardiomyocytes was observed using radioactive isotope tritium (3H)-leucine incorporation method. The effect of AngI on the expression of α-MHC and β-MHC genes in cardiomyocytes was analyzed by dot blot hybridization. RESULTS: The addition of Ang I to cultured cardiomyocytes significantly increased the incorporation of 3H-leucine into cardiomyocytes, and induced a rapid increase in the expression of α-MHC and β-MHC genes in cardiomyocytes. Both of them were present within a certain range. Dose-effect relationship. The AngI blocker sar-alasin blocks this effect of AngII. Conclusion: Ang II can increase the rate of protein synthesis in cardiomyocytes and promote the expression of α-MHC and β-MHC genes in cardiomyocytes.