论文部分内容阅读
我们知道要从肺结核病人的咯痰中直接找到结核杆菌的机会是比较少的,据称大约要每毫升痰液中含有结核杆菌100,000个以上才能用直接涂片找到。因此在涂片检查阴性的时候,我们常将标本浓缩处理,然后再作涂片检查,甚至还要接种培养基和豚鼠来碓定诊断。可是历来所用的浓缩法无非是用氢氧化钠(包括安替佛民法)或浓硫酸等将痰液消化,然后用离心器沉淀,取出沉渣做涂片检查。一般此法的检出率仍不高,因沉淀管内痰液的比重比较高,虽用每分钟3,000转的速度离心,结核杆菌仍不容易沉淀
We know that the chance of finding Mycobacterium tuberculosis directly from tuberculosis patients is relatively small, and it is reported that approximately 100,000 tuberculosis bacilli per milliliter of sputum should be found with a direct smear. Therefore, when the smear test is negative, we often concentrate the specimens, and then make smears, and even inoculate medium and guinea pigs to determine the diagnosis. However, the concentrating method used in the past is nothing more than the use of sodium hydroxide (including Antifod method) or concentrated sulfuric acid, etc., to digest the mash, and then use a centrifuge to precipitate, take out the sediment for smear inspection. In general, the detection rate of this method is still not high. Because the proportion of the phlegm in the sedimentation tube is relatively high, the tubercle bacillus is not easy to precipitate although it is centrifuged at a rate of 3,000 rpm.