论文部分内容阅读
目的:观察健择对大鼠肺组织的毒性和损伤,以及乌司他丁对肺损伤的保护作用。方法:雄性Wistar大鼠18只,随机分组为NS对照组、健择组(100mg/kg)和乌司他丁治疗组(10万U/kg),每组6只,分别静脉注射NS溶液(5mL/kg),健择(5mL/kg),乌司他丁溶液(10万U/kg)及健择(5mL/kg);2h后,股静脉注射EBD的NS溶液(浓度为1mg/mL,剂量为30mg/kg)。15min处死后分别测定左肺下叶湿干重比,右肺伊文思蓝OD值,左肺上叶观察肺组织病理改变。结果:3个实验组的动物体质量、左肺下叶湿重及单位体质量左肺重差异无统计学意义。但健择给药后,左肺湿/干重比显著高于对照组,而乌司他丁能拮抗健择的作用,使肺湿/干重比显著降低,至接近对照组水平。右肺依文思蓝的检测表明,健择组OD值增高,而乌司他丁组则与对照组接近,但由于个体差异大,各组之间的差异无统计意义。结论:健择可诱导大鼠肺损伤,但损伤较轻微,主要表现在肺组织血管通透性提高,渗出增加,引起肺湿/干重增加和肺组织依文思蓝量的增加。乌司他丁对健择引起的肺损伤有良好的对抗作用。
OBJECTIVE: To observe the toxicity and damage of gemcitabine on rat lung tissue and the protective effect of ulinastatin on lung injury. Methods: Eighteen male Wistar rats were randomly divided into three groups: NS control group, Jiangan group (100mg / kg) and ulinastatin group (100000U / kg) (5 mL / kg), gemcitabine (5 mL / kg), ulinastatin (100 000 U / kg) and gemcitabine (5 mL / kg) At a dose of 30 mg / kg). After 15min sacrifice, the left and right lower lobe wet and dry weight ratio, the right lung Evans blue OD value, the left lung upper lobe pathological changes were observed. Results: There were no significant differences in body weight, left lower lobe wet weight and left lung weight per unit body weight among the three experimental groups. However, after administration of gemcitabine, the left lung wet / dry weight ratio was significantly higher than the control group, while ulinastatin antagonized the role of gentamicin, lung wet / dry weight ratio was significantly reduced to close to the control group level. Right Evans blue test showed that the OD value of gemcitabine group increased, while the ulinastatin group was close to the control group, but due to individual differences, the differences between the groups were not statistically significant. CONCLUSION: Gemcitabine can induce lung injury in rats, but the lesion is minor, which is mainly manifested in the enhancement of vascular permeability, increased exudation of lung tissue, increased lung wet / dry weight and the increase of Evans blue in lung tissue. Ulinastatin has good antagonistic effect on the lung injury induced by gemcitabine.