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目的建立紫外光谱法研究2种离子液体(1-乙基-3-甲基咪唑-L-乳酸盐,1-丁基-3-甲基咪唑-L-乳酸盐)对β-环糊精与扑尔敏包合作用的影响。测定包合常数以及热力学常数,判断包合作用的推动力。方法测定不同浓度的β-环糊精中加入扑尔敏后的吸光度值。以1/A对1/[CD]0作图,根据斜率及截距求得结合常数K;考察了温度以及离子液体对包合作用的影响。结果β-环糊精对扑尔敏的包合量比为1∶1,结合常数随温度的升高而增加,消旋扑尔敏的包合常数稍大于右旋扑尔敏的包合常数。加入离子液体之后,消旋扑尔敏的包合常数明显增加,而且1-丁基-3-甲基咪唑-L-乳酸盐比1-乙基-3-甲基咪唑-L-乳酸盐对包合常数的影响大。结论得到的热力学常数表明β-环糊精对扑尔敏的包合作用是一个焓变、熵变的共同驱动的过程,疏水作用为主要的驱动力。本文的实验结果为毛细管电泳中使用β-环糊精和离子液体拆分扑尔敏提供了理论依据。
Objective To establish a UV spectrophotometric method for the determination of β-cyclodextrin in 2 kinds of ionic liquids (1-ethyl-3-methylimidazolium-L-lactate, 1-butyl- Effect of fine and chlorpheniramine inclusion. Determination of inclusion constants and thermodynamic constants, to determine the inclusion of the driving force. Methods The absorbance values of chlorpheniramine in different concentrations of β-cyclodextrin were determined. The 1 / [CD] 0 was plotted with 1 / A, and the binding constant K was obtained from the slope and the intercept. The effects of temperature and ionic liquid on inclusion were investigated. Results The inclusion ratio of β-cyclodextrin to chlorpheniramine was 1: 1, and the binding constant increased with the increase of temperature. The inclusion constant of racemic chlorpheniramine was slightly larger than that of dextro-chlorpheniramine . After addition of ionic liquid, the inclusion of racemic chlorpheniramine significantly increased, and 1-butyl-3-methylimidazolium-L-lactate was more stable than 1-ethyl-3- Salt on the inclusion of a large impact. Conclusion The thermodynamic constants obtained indicate that inclusion of β-cyclodextrin to chlorpheniramine is a co-driven process of enthalpy change and entropy change, and hydrophobicity is the main driving force. The experimental results in this paper provide a theoretical basis for the resolution of chlorpheniramine using β-cyclodextrin and ionic liquids in capillary electrophoresis.