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目的:探讨虫草多糖(Cordyceps Polysaccharides,CP)对小鼠皮肤成纤维细胞抗氧化能力的影响。方法:用8-甲氧补骨脂素联合紫外线(8-MOP/UVA)制备皮肤成纤维细胞氧化模型,通过检测细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶GSH-Px含量反映细胞内自由基含量及清除能力,并测定线粒体跨膜电位的破坏情况判断细胞线粒体功能。结果:模型组细胞的自由基含量较对照组增多,自由基清除酶系统活力下降,虫草多糖保护组的自由基含量较模型组低,自由基清除能力也有所改善;线粒体跨膜电位的破坏程度也有所改善。结论:虫草多糖能增加皮肤成纤维细胞的抗氧化能力。
Objective: To investigate the effect of Cordyceps Polysaccharides (CP) on the anti-oxidation ability of mouse skin fibroblasts. Methods: The skin fibroblasts oxidation model was established by 8-methoxypsoralen combined with ultraviolet (8-MOP / UVA). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) The content of glutathione peroxidase (GSH-Px), CAT and GSH-Px in the cells reflected the intracellular free radical content and scavenging capacity. The damage of mitochondrial transmembrane potential was also measured to determine the mitochondrial function. Results: The content of free radicals in the model group was higher than that in the control group, and the activity of free radical scavenging enzyme system was decreased. The content of free radicals in the protective group of Cordyceps polysaccharide was lower than that of the model group, and the free radical scavenging ability was also improved. The destruction degree of mitochondrial transmembrane potential It has also improved. Conclusion: Cordyceps polysaccharide can increase the anti-oxidative capacity of skin fibroblasts.