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用培养的原代心肌细胞建立缺氧－再给氧方法，对缺氧６０分钟、１２０分钟及缺氧后再给氧３０及６０分钟时心肌细胞搏动、ＬＤＨ活性、（５１）￣Ｃｒ释放率、苔盼蓝摄取率、扫描电镜观察及１，６－二磷酸果糖对其影响进行了研究。结果显示：缺氧２小时可引起心肌细胞搏动频率减慢，再给氧后搏动频率加快；ＬＤＨ活性、（５１）￣Ｃｒ释放率和苔盼蓝摄取率增加，电镜显示心肌细胞膜受损。１，６－二磷酸果糖对心肌细胞膜具有保护作用。
The hypoxia-reoxygenation method was established by culturing primary cardiomyocytes. The cardiomyocyte pulsatility, LDH activity, (51) Cr release rate at 60 minutes, 120 minutes and 30 minutes and 60 minutes after anoxia were also observed. , Trypan blue uptake rate, scanning electron microscopy and fructose-1,6-diphosphate were studied. The results showed that hypoxia for 2 hours caused a decrease in the frequency of cardiomyocytes pulsation and accelerated pulsation after oxygen administration. The LDH activity, (51) Cr release rate and the increase of trypan blue increased, and myocardial cell membrane was damaged by electron microscopy. 1,6-diphosphate fructose on myocardial cell membrane has a protective effect.