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水稻和稻瘟病菌互作是研究植物与病原菌互作的模式体系。本文利用3个水稻抗稻瘟病近等基因品系(CO39、C101LAC和C101A51)和2个特异性菌株(M209和M210)构成不同亲和程度的互作关系,研究了稻瘟病菌对3个水稻信号传导途径关键酶合成基因、5个防御反应病程相关蛋白基因和1个防御反应转录因子调控基因诱导表达的作用。结果表明:稻瘟病菌诱导了各种互作关系中水稻OsLOX、OsAOS和OsPAL酶合成基因的表达,水稻启动了茉莉酸和水杨酸防御反应信号传导途径。在不亲和的互作反应中,稻瘟病菌能不同程度地诱导水稻OsPR1a、OsPR2、OsPR3-1、OsPR3-2和OsPR4基因的表达,从而有效激活了防御反应系统,使水稻植株表现为抗病;而在亲和的互作反应中,多数OsPR基因的表达水平低、时间短或没有表达,水稻植株表现为感病。OsMyb基因在各种互作关系中有不同的诱导表达。说明这些防御相关基因的诱导表达可能与水稻抗稻瘟病性相关。
Interaction between rice and Magnaporthe grisea is a model system for studying the interaction between plants and pathogenic bacteria. In this study, three rice blast-resistant proximal isogenic lines (CO39, C101LAC and C101A51) and two specific strains (M209 and M210) were used to construct interactions with different affinity levels. The key enzyme synthesis pathway of conduction pathway, the expression of five defense-responsive disease-related protein genes and a defense-responsive transcription factor-regulated gene. The results showed that Magnaporthe grisea induced the expression of rice OsLOX, OsAOS and OsPAL synthase genes in various interactions, and rice started the defense response signal transduction pathway of jasmonic acid and salicylic acid. In incompatible interactions, M. grisea induced the expression of OsPR1a, OsPR2, OsPR3-1, OsPR3-2 and OsPR4 genes in rice to some extent, which effectively activated the defense response system and made the rice plants resistant However, most of the OsPR genes were expressed at low or short time or not in the reciprocal interaction, and the rice plants showed susceptibility. The OsMyb gene has different inducible expression in various interactions. Indicating that the induced expression of these defense-related genes may be related to rice blast resistance.