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为了解空间诱变产生的水稻核雄性不育突变体ws-3-1基因表达的特点,对ws-3-1和原种特籼占13减数分裂二分体时期的cDNA进行了抑制缩减杂交(SSH)分析,分别从正向和反向缩减文库中检测到109和104个表达有差异的克隆.对这些克隆进行了测序和同源性分析,并利用Northern杂交技术,从中鉴定出5个在原种与突变体间减数分裂二分体时期表达有差异的基因,并分析了该雄性不育突变体基因表达的变化与表型的关系.
In order to understand the characteristics of ws-3-1 gene expression induced by space mutagenesis in rice nuclear male sterility mutant, the cDNA of ws-3-1 and the original seed of indica accounted for 13 meiotic lysis were reduced Hybridization (SSH) analysis, 109 and 104 differentially expressed clones were detected respectively from the forward and reverse subtracted libraries.These clones were sequenced and homolog analyzed and Northern blotting was used to identify 5 The results showed that there was a significant difference in gene expression between the elite and the mutant during meiosis. The relationship between the gene expression and the phenotype was analyzed.