论文部分内容阅读
对７２ 株临床分离的绿脓杆菌进行了药敏试验， 其中４２ 株检测了β－内酰胺酶及几种β－内酰胺酶基因同源序列在总ＤＮＡ和质粒中的情况，并探讨了绿脓杆菌总ＤＮＡ中不同β－内酰胺酶基因同源序列与不同β－内酰胺类抗生素耐药性的关系。结果显示， 绿脓杆菌有普遍耐药性和多重耐药性， 在４２ 株绿脓杆菌中有３４ 株产β－内酰胺酶， 阳性率８０．９５％ ， β－内酰胺酶基因同源序列在总ＤＮＡ中的检出亦有普遍性和多重性的特点， ４２ 株菌总ＤＮＡ 中检出ＰＳＥ－１、ＴＥＭ－１、ＳＨＶ－２、ＯＸＡ－１、ＯＸＡ－２β－内酰胺酶基因同源序列２～４种不等， 其中有１７ 株菌分离到质粒（１７／４２）， 这１７株菌的质粒均检出１～２ 种β－内酰胺酶基因同源序列不等。从酶基因的类型看，染色体介导的以ＰＳＥ－１型为主，质粒介导的以ＴＥＭ－１ 型为主。Ｌｏｇｉｓｔｉｃ回归分析显示β－内酰胺类抗生素耐药性与β－内酰胺酶基因的关系有３ 种情况： （１） 与一种特定的β－内酰胺酶基因有关； （２） 与几种β－内酰胺酶基因有关； （３） 与β－内酰胺酶基因无关。
72 clinical isolates of Pseudomonas aeruginosa drug susceptibility testing, of which 42 were detected β-lactamase and several β-lactamase gene homologous sequences in the total DNA and plasmid situation, and to explore the green Relationship between different β-lactamase gene homologous sequences and drug resistance of different β-lactam antibiotics in total DNA of Pseudomonas aeruginosa. The results showed that Pseudomonas aeruginosa has universal resistance and multi-drug resistance, 34 strains of Pseudomonas aeruginosa produce β-lactamase, the positive rate of 80.95%, β-lactamase gene homologous sequence The detection of total DNA was also common and multiplicity characteristics, 42 strains of bacteria found in the total DNA PSE-1, TEM-1, SHV-2, OXA-1, OXA-2β-lactamase gene with Among the 17 strains, 17 strains isolated from plasmids (17/42). The plasmids of these 17 strains all detected 1 ~ 2 homologous sequences of β-lactamase genes. From the type of enzyme genes, the chromosome-mediated PSE-1-based, plasmid-mediated TEM-1-based. Logistic regression analysis showed that β-lactam antibiotic resistance and β-lactamase gene relationship in three cases: (1) with a specific β-lactamase gene; (2) with several β - lactamase gene; (3) and β-lactamase gene has nothing to do.