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目的:基于钙激活氯离子通道探讨电针足三里、梁门和三阴交治疗糖尿病胃轻瘫(DGP)的作用机制.方法:将40只Sprague-Dawley大鼠随机分为4组,即空白对照组(A组)、模型组(B组)、电针组(C组)和胃复安组(D组),每组10只.采用单次腹腔注射2％的链脲佐菌素(STZ)和8周高糖高脂饮食建立DGP大鼠模型.干预后测定胃肠推进率,免疫组化检测跨膜蛋白16A(TMEM16A)的表达水平,免疫荧光法检测Cajal间质细胞(ICCs)内Ca2+浓度,应用全细胞膜片钳技术检测胃窦ICCs中钙激活氯离子通道电流强度(ICaCC).结果:造模后,B、C和D组血糖较A组明显升高(均P<0.01);干预后,C和D组血糖较B组明显降低(P<0.05,P<0.01);造模后与干预后组内血糖比较,仅C组具有统计学意义(P<0.01).B、C和D组胃肠推进率与A组差异具有统计学意义(P<0.01或P<0.05);C和D组胃肠推进率明显高于B组(P<0.01,P<0.01).B和C组的TMEM16A表达较A组降低(P<0.01,P<0.05);C和D组TMEM16A的表达较B组升高(P<0.01,P<0.05).B组Ca2+荧光强度明显低于A组(P<0.01),C和D组Ca2+荧光强度明显高于B组(P<0.01,P<0.05).B组ICCs内ICaCC较A组明显降低,C、D组电流较B组升高.结论:电针足三里、梁门和三阴交可通过上调ICCs内ICaCC改善DGP大鼠的胃肠动力.“,”Objective: To investigate the mechanisms of electroacupuncture (EA) at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) in intervening diabetic gastroparesis (DGP) based on calcium-activated chloride channel. Methods: Forty Sprague-Dawley rats were randomly divided into four groups, including a normal control group (group A), a model group (group B), an EA group (group C) and a metoclopramide group (group D), with 10 rats in each group. A single intraperitoneal injection of 2％ streptozotocin (STZ) combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model. After intervention, gastrointestinal propulsive rate was observed; the expression level of transmembrane protein 16A (TMEM16A) was examined by immunohistochemistry; the Ca2+ concentration in interstitial cells of Cajal (ICCs) was detected by immunofluorescence; and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel (ICaCC) in ICCs in gastric antrum. Results: After modeling, the blood glucose levels in group B, group C and group D were significantly increased compared with group A (all P<0.01); after intervention, compared with group B, the blood glucose levels in group C and group D were significantly decreased (P<0.05, P<0.01); the intra-group comparison of blood glucose level between after modeling and after intervention found significant difference only in group C (P<0.01). The gastrointestinal propulsive rates in group B, group C and group D were significantly different from that in group A (P<0.01 or P<0.05); the gastrointestinal propulsive rates were markedly higher in group C and group D than in group B (P<0.01, P<0.01). The expressions of TMEM16A in group B and group C were decreased compared with group A (P<0.01, P<0.05); the expressions of TMEM16A in group C and group D were increased compared with group B (P<0.01, P<0.05). The fluorescence intensity of Ca2+ was significantly lower in group B than in group A (P<0.01); the fluorescence intensity of Ca2+ was significantly higher in group C and group D than in group B (P<0.01, P<0.05). ICaCC in ICCs in group B was significantly decreased compared with group A; ICaCC in group C and group D were increased compared with group B. Conclusion: EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can significantly improve gastrointestinal motility in DGP rats by up-regulating the ICaCC in ICCs.