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目的本研究探讨了肿瘤抗原触发的Ｍ－ＣＳＦ和ＩＦＮ－γ基因联合转染的巨噬细胞对黑色素瘤实验性肺转移的治疗作用及其免疫机理。方法将联合基因转染的巨噬细胞经尾静脉回输治疗黑色素瘤实验性肺转移小鼠，经治疗后于荷瘤后第１７天处死小鼠，计数荷瘤小鼠的肺部转移结节数并观察其长期存活期，用５１Ｃｒ４小时释放法检测体外诱导的ＣＴＬ活性，采用未配对计量资料的ｔ检验处理数据。结果联合基因转染后治疗组的小鼠长期存活率为１６％，加用肿瘤抗原触发后的治疗组有３３％的小鼠长期存活。小鼠脾细胞经体外诱导的ＣＴＬ对野生型Ｂ１６．Ｆ１０细胞的杀伤活性，在经肿瘤抗原触发的Ｍ－ＣＳＦ和ＩＦＮ－γ联合基因转染巨噬细胞治疗的荷瘤小鼠最高，与用未经抗原触发的联合基因转染巨噬细胞治疗组差异有显著性（Ｐ＜０．０５）。结论体外经肿瘤抗原触发的Ｍ－ＣＳＦ、ＩＦＮ－γ联合基因转染的巨噬细胞过继回输疗法是肿瘤特别是转移性肿瘤的一种有效免疫治疗手段，机体的ＣＴＬ在该疗法的抗肿瘤作用中可能发挥了重要的作用。
Objective This study was to investigate the therapeutic effect of macrophages transfected with tumor antigen-triggered M-CSF and IFN-γ genes on experimental lung metastasis of melanoma and its immune mechanism. Methods Transfected macrophages were treated with tail vein to treat mice with experimental lung metastasis of melanoma. After treatment, mice were sacrificed on the 17th day after tumor implantation. Tumor metastasis nodules were counted in tumor-bearing mice. The long-term survival time was also observed and the CTL activity induced in vitro was detected by 51Cr 4-hour release method. The data was processed by t-test with unpaired measurement data. Results The long-term survival rate of the mice treated with the combined gene transfection group was 16%, and 33% of the mice treated with the tumor antigen-triggered group survived for a long time. Mouse splenocytes were induced in vitro by CTL versus wild-type B16. The killing activity of F10 cells was highest in tumor-bearing mice treated with tumor antigen-activated M-CSF and IFN-γ combined gene-transfected macrophages and transfected with a combination of non-antigen-triggered macrophage therapy The difference was significant (P < 0.05). CONCLUSION: In vitro reinfusion of macrophages transfected with tumor antigen-activated M-CSF and IFN-gamma-associated genes is an effective immunotherapy for tumors, especially metastatic tumors. The body’s CTL is antitumor in this therapy. The role may play an important role.