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目的从ＤＮＡ水平揭示ｎｍ２３－Ｈ１杂合性基因丢失与人原发性肝癌肝内转移、门静脉形成癌栓等的临床病理特征的关系。方法应用Ｓｏｕｔｈｅｒｎ印迹杂交技术，对２５例肝癌及癌旁肝组织ＤＮＡ进行分析。结果ｎｍ２３－Ｈ１两条等位基因分别为７．６Ｋｂ、２．３Ｋｂ，杂合性等位基因丢失率为３１．２５％（５／１６）。ｎｍ２３－Ｈ１杂合性基因丢失多见于分化较差的ＥｄｍｏｎｄｓｏｎⅢ、Ⅳ级和伴肝内转移或门静脉形成癌栓的肝癌。结论ｎｍ２３－Ｈ１可能诱发肝细胞肝癌的转移潜能，有助于预测肝癌复发和转移。
Objective To reveal the relationship between the loss of heterozygosity of nm23-H1 gene and the clinicopathological features of human primary hepatocellular carcinoma, such as intrahepatic metastasis and portal vein tumor thrombosis. Methods Southern blot hybridization was used to analyze the DNA of 25 cases of hepatocellular carcinoma and adjacent liver tissues. Results The two alleles of nm23-H1 were 7.6 Kb and 2.3 Kb, respectively. The loss rate of heterozygous allele was 31.25% (5/16). The loss of heterozygosity of nm23-H1 was more common in poorly differentiated Edmondson III, IV, and hepatocellular carcinoma with intrahepatic metastasis or portal vein thrombosis. Conclusion nm23-H1 may induce the metastatic potential of hepatocellular carcinoma, and it is helpful to predict the recurrence and metastasis of hepatocellular carcinoma.