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鉴定和克隆水稻穗顶部退化突变体新基因,对研究小穗顶部退化的分子机制及克服育种和生产实践中因穗顶部退化引起的产量损失具有重要的理论和现实意义。本研究报道了一个来源于中花11的穗顶部退化突变体,暂命名为Panicle Apical Abortion 2(paa2)。该突变体的穗顶部小穗发育异常、退化,后期退化部分脱落,稻穗形成秃尖,使穗粒数减少。遗传分析表明该突变体受1个显性基因控制。利用群体分离分析法(BSA,bulked segregation analysis)将PAA2基因定位在2号染色体的长臂端L2-33和L2-50之间,物理距离为80 kb的范围内。该研究结果为PAA2基因的图位克隆奠定了基础。
Identifying and cloning the new gene of top degenerative mutant in rice has important theoretical and practical significance for studying the molecular mechanism of top degeneration in spikelet and overcoming the yield loss caused by top degeneration in breeding and production practice. This study reported a panicle degeneration mutant derived from Zhonghua 11, tentatively named Panicle Apical Abortion 2 (paa2). The spikelet at the top of panicle of this mutant developed abnormally, degenerated, partially degenerated at later stage, and bald tip of panicle formed, which reduced the number of spikelets per panicle. Genetic analysis showed that the mutant was controlled by a dominant gene. The PAA2 gene was located on the long arm of chromosome 2 between L2-33 and L2-50 with a physical distance of 80 kb using bulked segregation analysis (BSA). The results laid the foundation for the map-based cloning of PAA2 gene.