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本研究以粳稻品种“藤坂5号”与籼稻品种“江西丝苗”为亲本杂交构建的F2分离群体(137个株系)为作图群体,对F2:3家系的3叶期水稻幼苗冷处理(10℃/8℃,昼/夜)5 d、恢复培养7 d后的耐冷级别、叶枯萎度及苗成活率进行完备区间作图。在分子标记连锁分析过程中共检测到6个控制水稻耐冷性相关性状的QTLs,其中,控制耐冷级别、叶枯萎度及苗成活率的QTLs各有2个,而且每个性状的2个QTLs都分别定位在第8染色体的RM22772-C61344和第11染色体的M100-RM26567区域内,这些QTLs具有较高的耐冷表型贡献率(14.09%~28.60%)。QTL的定位结果采用置换检验(Permutation test)进行了验证,发现控制耐冷级别的2个QTLs的F值都超过了QTL检测的阈值,而叶枯萎度和苗成活率2个性状的QTLs中各有1个QTL(q LWR-8和q SR-11)的F值超过了QTL检测的阈值。研究结果显示在第8和11染色体确实存在2个与水稻耐冷性相关的位点,可为进一步的分析和精细定位打下基础,也为耐冷水稻品种育种和种质创新提供理论依据。
In this study, F2 segregating population (137 lines) constructed from the cross between japonica rice “Fujisaka 5 ” and indica rice “Jiangxi silk seedling ” was used as the mapping population. Cold tolerance of the seedlings (10 ℃ / 8 ℃, day / night) for 5 days and recovery of cold tolerance after 7 days of culture, leaf blight and seedling survival rate were plotted. Six QTLs controlling cold tolerance traits were detected in molecular marker linkage analysis. Among them, two QTLs controlling cold tolerance, leaf blight and seedling survival rate were 2, and 2 QTLs for each trait These QTLs were located in RM22772-C61344 on chromosome 8 and M100-RM26567 on chromosome 11, and these QTLs had a higher contribution rate of cold-resistant phenotype (14.09% -28.60%). The results of QTL mapping were validated by Permutation test and found that the F values of two QTLs controlling cold tolerance exceeded the QTL detection thresholds, and the QTLs of leaf blight and seedling survival rate had two The F value of one QTL (q LWR-8 and q SR-11) exceeded the QTL detection threshold. The results show that there are two loci related to cold tolerance in chromosomes 8 and 11, which may lay the foundation for further analysis and fine mapping, and provide a theoretical basis for breeding and germplasm innovation of cold-tolerant rice varieties.