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目的建立兔眼视网膜母细胞瘤(Rb)动物模型,便于和扩展应用眼肿瘤动物模型进行临床实验治疗。设计实验性研究。研究对象8只兔(16眼)及HXO-Rb44细胞株。方法全麻下在兔眼脉络膜上腔注入浓度为(1．17×107)／ml肿瘤细胞悬液0．1ml,分别于种植后的l、3、7、14天观察记录兔眼底改变,第14天摘除眼球后行病理观察和肿瘤细胞鉴定。主要指标观察肿物生长数量及生长方式,并以病理切片H-E和免疫组化染色证实细胞来源。结果种植后1周肿物开始生长,生长方式有肿块状和弥漫状,成活率为7／16。肿瘤细胞生长活跃,生长旺盛区与坏死区相间隔;细胞呈未分化的特点:核大深染,胞浆少,核与胞浆比例失调,异形性明显,并见大量的核丝分裂。经H-E和免疫组化染色,证明兔眼Rb的生长方式、分化类型及细胞特征与人眼Rb相一致。结论HXO-Rb44细胞株可以在兔眼内成活,并能形成实体性肿瘤,兔可以成为较理想的Rb模型动物。
Objective To establish an animal model of retinoblastoma (Rb) in rabbits and to facilitate and expand the animal model of ocular tumor for clinical experimental treatment. Design experimental research. The study included 8 rabbits (16 eyes) and HXO-Rb44 cell lines. Methods Under anesthesia, 0.1ml of tumor cell suspension (1.17 × 107) / ml was injected into the suprachoroidal space of rabbit eyes, and the change of fundus fundus was recorded on days 1, 3, 7 and 14 after implantation. 14 days after removal of the eye pathological observation and tumor cell identification. The main indicators observed the number of tumor growth and growth patterns, and pathological sections of H-E and immunohistochemical staining confirmed cell origin. Results One week after planting, the tumor started to grow, and the growth pattern was massive and diffuse, with a survival rate of 7/16. Tumor cells grow actively, and the area with strong growth is separated from the necrotic area. The characteristics of the cells are undifferentiated: the nucleus is deeply stained, the cytoplasm is less, the ratio of nucleus to cytoplasm is imbalanced, and the abnormality is obvious. H-E and immunohistochemical staining showed that the growth pattern, the type of differentiation and the cell characteristics of Rb in rabbit eyes were consistent with human Rb. Conclusion The HXO-Rb44 cell line can survive in the rabbit eye and form a solid tumor. Rabbit can be an ideal Rb model animal.