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目的探讨链脲佐菌素(STZ)诱导的糖尿病小鼠胸腺中CD4+CD25+调节性T(Tr)细胞数的变化。方法将40只健康昆明小鼠随机分为4组,每组各10只。Ⅰ组小鼠腹腔注射枸橼酸钠缓冲液(0.2mL/只)作为对照;Ⅱ、Ⅲ、Ⅳ组小鼠分别腹腔注射20、40、80mg/kg的STZ连续5d,每天1次。用尿糖试纸检测小鼠尿糖。用葡萄糖测定试剂盒检测小鼠血糖。用间接ELISA法检测小鼠自身抗体的水平。用流式细胞术检测胸腺中Tr细胞数的变化。结果Ⅱ、Ⅲ组小鼠血糖、自身抗体的水平明显高于对照组(P<0.05),胰岛中有淋巴细胞浸润,胸腺中Tr细胞数低于对照组。结论STZ可诱导小鼠产生糖尿病,但不同剂量的STZ所引起的胰腺和胸腺的病理变化不同。其中,中等剂量的STZ既可诱导小鼠发生糖尿病,又可使胸腺中Tr细胞数减少。后者可能与小鼠自身免疫性糖尿病的发生有关。
Objective To investigate the changes of CD4 + CD25 + regulatory T (Tr) cells in thymus of streptozotocin (STZ) -induced diabetic mice. Methods Forty healthy Kunming mice were randomly divided into 4 groups with 10 mice in each group. The mice in group Ⅰ were injected intraperitoneally with sodium citrate buffer solution (0.2mL / mouse) as control group; the mice in group Ⅱ, Ⅲ and Ⅳ were injected intraperitoneally with 20,40,80mg / kg STZ for 5 days continuously. Using urine test strips to detect urine glucose in mice. Mice blood glucose was measured with glucose assay kit. Indirect ELISA was used to detect the levels of autoantibodies in mice. The changes of Tr cells in thymus were detected by flow cytometry. Results The levels of blood glucose and autoantibodies in mice in groups Ⅱ and Ⅲ were significantly higher than those in the control group (P <0.05). There was lymphocyte infiltration in the islets and the number of Tr cells in the thymus was lower than that in the control group. Conclusion STZ can induce diabetes in mice, but different doses of STZ caused different pathological changes in the pancreas and thymus. Among them, moderate doses of STZ can induce diabetes in mice, but also reduce the number of Tr cells in the thymus. The latter may be related to the occurrence of autoimmune diabetes in mice.