Plant regeneration from cultured protoplasts of a glutinous rice

• 出 处: Cell Research
• 发表日期: 1990年01期
• 关键词: cultured regeneration cytoplasm inducing subsp agarose mature dense xiang Biotec

Young embryos of rice (Oryza sativa L. subsp, japonicavar. Guo-xiang No. 1) were cultured on MS agar medium(2,4-D 2 mg/l). Calli were formed and subcultured on N6 agar medium (2,4-D 2 mg/l ). After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium (2,4-D 1 mg/l, 6-BA 0.2 mg/l)~1 with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.

Calli were formed and subcultured on a N6 agar medium (2, 4-D 2 mg / 4-D 2 mg / 1). After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium (2,4-D 1 mg / BA 0.2 mg / l) ~ 1 with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.