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目的:为获得癫痫患者免疫学异常的证据,本实验检测了患者血清中抗脑抗体(Anti-encephalic antibodies,AEAb)及脑组织人类白细胞抗原Ⅱ类抗原(Human leukocyte antigen class Ⅱantigen,HLA class Ⅱ antigen,HLA-Ⅱ类抗原),并与正常对照组比较。对象及方法:1.用ELISA方法测定37例癫痫患者血清抗脑自身抗体;2.借助免疫组织化学方法观察了HLA—Ⅱ类抗原在脑组织中表达与分布。结果:1.癫痫患者血清抗脑抗体高于正常对照;2.癫痫患者脑组织中星形胶质细胞和小胶质细胞异常表达HLA—Ⅱ类抗原。讨论:本实验证实癫痫患者存在一定程度的自身免疫应答异常。脑组织胶质细胞表面HLA—Ⅱ类抗原表达异常可能通过多种机制参与癫痫发病。
OBJECTIVE: To obtain evidence of immunological abnormalities in patients with epilepsy, we tested the serum anti-encephalic antibodies (AEAb) and human leukocyte antigen class Ⅱ antigen (HLA class Ⅱ antigen , HLA-Ⅱantigen), and compared with the normal control group. PARTICIPANTS AND METHODS: 1. Serum anti-brain autoantibodies were measured in 37 epileptic patients by ELISA; 2. The expression and distribution of HLA-Ⅱantigen in brain tissue were observed by immunohistochemistry. Serum anti-brain antibodies in patients with epilepsy were higher than those in normal controls.2. Astrocytes and microglia in brain tissue of patients with epilepsy were abnormally expressed HLA class II antigens. Discussion: This experiment confirmed that there is a certain degree of abnormalities in autoimmune response in patients with epilepsy. Abnormal expression of HLA class II antigens on the surface of glial cells may be involved in the pathogenesis of epilepsy through various mechanisms.