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目的研究外源NO对NaCl胁迫下水飞蓟种子萌发、幼苗生长生理的影响。方法用0.05～0.60 mmol/L亚硝基铁氰化钠(SNP)溶液浸种,在0.7%NaCl胁迫下发芽,观察种子发芽势、发芽率、发芽指数和活力指数。并观察幼苗生长情况,检测叶片中丙二醛(MDA)、光合色素和渗透性物质量,以及保护酶活性。结果 0.7%NaCl胁迫下,水飞蓟种子萌发和幼苗生长受到显著抑制,用0.05～0.60 mmol/L SNP溶液浸种能促进水飞蓟种子萌发和幼苗生长,降低叶片中MDA量,增加光合色素(叶绿素a、叶绿素b、叶绿素总量和类胡萝卜素)和渗透性物质(可溶性糖、可溶性蛋白和脯氨酸)量,提高保护酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)]活性。结论 0.05～0.60 mmol/L SNP浸种能提高水飞蓟种子和幼苗的抗盐能力。不同栽培类型水飞蓟对SNP敏感度不同,其中,0.10 mmol/L SNP对白色种皮处理效果最好;0.40 mmol/L SNP对黑色种皮处理效果最好。
Aim To study the effects of exogenous nitric oxide (NO) on seed germination and seedling growth of Silybum Marianas under NaCl stress. Methods Soaked seeds with 0.05-0.60 mmol / L SNP solution and germinated under 0.7% NaCl stress, the seed germination potential, germination rate, germination index and vigor index were observed. The growth of seedlings was observed. The contents of malondialdehyde (MDA), photosynthetic pigments and osmolytes, and the activity of protective enzymes in leaves were measured. Results Seeds germination and seedling growth were significantly inhibited under the stress of 0.7% NaCl. Seeding with 0.05-0.60 mmol / L SNP solution could promote seed germination and seedling growth, reduce MDA content in leaves, increase photosynthetic pigment ( Chlorophyll a, chlorophyll b, total chlorophyll and carotenoids), and the amount of osmolytes (soluble sugar, soluble protein and proline) increased the activities of protective enzymes [superoxide dismutase (SOD), peroxidase ) And catalase (CAT)] activity. Conclusion 0.05 ~ 0.60 mmol / L SNP soaking can improve the salt tolerance ability of silymarin seeds and seedlings. Silybum marianum var. Sibiricum had different sensitivity to SNP, of which 0.10 mmol / L SNP had the best effect on white seed coat and 0.40 mmol / L SNP had the best effect on black seed coat.