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利用分子印迹技术与表面等离子共振光谱联用,建立了对磺酰脲类除草剂烟嘧磺隆的检测方法.实验过程中,探讨了pH值对分子印迹膜吸附目标化合物特性的影响,并在最佳pH下对其吸附和选择性能进行了评价.与非印迹聚合物相比,烟嘧磺隆印迹聚合物对烟嘧磺隆吸附效率比烟嘧磺隆类似物高.该方法的线性范围为5.0×10-12～25×10-12 mol/L,烟嘧磺隆和分子印迹聚合物之间的结合常数为1.6×1010 L/mol,吉布斯自由能变化值为-58.148 kJ/mol.研究结果表明该方法具有简单、快速、灵敏度高、重复性好等特点,适用于自来水和土壤中磺酰脲类除草剂烟嘧磺隆的测定,基于信噪比为3时,自来水和土壤的检出限分别为5.62×10-14和1.01×10-13 mol/L,平均回收率分别为85.6%和76.6%,相对标准偏差分别为1.78%和3.21%.
The method of detecting sulfonylurea herbicide nicosulfuron was established by using molecular imprinting technique and surface plasmon resonance spectroscopy.The influence of pH value on the characteristics of target compounds adsorbed by molecularly imprinted membrane Its adsorption and selectivity at the optimum pH were evaluated.Compared with non-imprinted polymers, nicosulfuron imprinted polymers showed higher adsorption efficiency for nicosulfuron than nicosulfuron analogs.The linear range Was 5.0 × 10-12 ~ 25 × 10-12 mol / L, the binding constant of nicosulfuron and molecularly imprinted polymer was 1.6 × 1010 L / mol, Gibbs free energy was -58.148 kJ / mol. The results show that the method has the characteristics of simple, rapid, high sensitivity and good repeatability. It is suitable for the determination of sulfonylurea herbicide nicosulfuron in tap water and soil. Based on the signal to noise ratio of 3, The detection limits of soil were 5.62 × 10-14 and 1.01 × 10-13 mol / L, respectively. The average recoveries were 85.6% and 76.6%, respectively, and the relative standard deviations were 1.78% and 3.21% respectively.