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目的:探讨牡蛎肽对免疫抑制小鼠的免疫调节作用及可能机制。方法:以环磷酰胺(cyclophosphamide,CTX)致免疫低下雌性ICR小鼠为动物模型,观察不同剂量牡蛎肽(0.5 g/kg低剂量组、1.0 g/kg中剂量组、2.0 g/kg高剂量组)干预15 d后对小鼠体重、免疫器官相对重量、免疫器官显微结构、外周血白细胞数目、T淋巴细胞亚群百分比、血清中的细胞因子浓度以及骨髓有核细胞数和DNA含量的影响。结果:腹腔注射CTX模型组小鼠与对照组相比,胸腺和脾脏萎缩,显微结构紊乱,外周血白细胞数目降低(P=0.04),外周血CD3+T淋巴细胞百分比下降(P=0.003),CD8+T淋巴细胞百分比下降(P=0.002),外周血中IL-5浓度显著升高(P<0.01),小鼠骨髓有核细胞浓度及骨髓DNA含量降低(P=0.04,P<0.01)。牡蛎肽灌胃组小鼠的胸腺和脾脏显微结构恢复正常,高剂量牡蛎肽灌胃组小鼠的外周血白细胞数目与模型组相比升高(P=0.003),中剂量(P=0.04)和高剂量(P=0.02)的牡蛎肽灌胃组小鼠外周血CD3+T淋巴细胞百分比与模型组相比升高,高剂量的牡蛎肽灌胃组小鼠外周血CD8+T淋巴细胞百分比与模型组相比升高(P=0.002),各剂量牡蛎肽灌胃组的小鼠外周血中IL-5浓度与模型组小鼠相比显著降低(P值均<0.01),高剂量牡蛎肽灌胃组的小鼠外周血中IL-17浓度与对照组相比明显降低(P=0.03),各剂量牡蛎肽灌胃组的小鼠骨髓有核细胞浓度与模型组相比均明显升高(低剂量组vs.模型组,P=0.04;中剂量组vs.模型组,P=0.02;高剂量组vs.模型组,P=0.01),小鼠骨髓DNA含量与模型组相比也均明显升高(P值均<0.01)。结论:牡蛎肽可改善CTX所致免疫低下小鼠紊乱的免疫器官结构,恢复CTX造成的小鼠T淋巴细胞比例失调及细胞因子紊乱,升高骨髓有核细胞数及骨髓DNA含量,从而增强机体免疫功能。
Objective: To investigate the immunomodulatory effect of oyster peptide on immunosuppressed mice and its possible mechanism. Methods: The immunosuppressive female ICR mice induced by cyclophosphamide (CTX) were used as animal models. The effects of different doses of oyster peptide (0.5 g / kg low dose group, 1.0 g / kg middle dose group, 2.0 g / kg high dose Group) on the weight of mice, the relative weight of immune organs, the microstructure of immune organs, the number of leukocytes in peripheral blood, the percentage of T lymphocyte subsets, the concentration of cytokines in serum and the number of nucleated cells and DNA in bone marrow influences. Results: Compared with the control group, the thymus and spleen atrophied, the microstructure was disorganized, the number of leukocytes in peripheral blood decreased (P = 0.04) and the percentage of CD3 + T lymphocytes in peripheral blood decreased (P = 0.003) , The percentage of CD8 + T lymphocytes decreased (P = 0.002), the concentration of IL-5 in peripheral blood increased significantly (P <0.01), the concentration of bone marrow cells and the content of bone marrow decreased ). The microstructure of thymus and spleen of oyster peptide-gavage mice returned to normal, and the number of peripheral leukocytes in high-dose oysterin-administered mice increased compared with model group (P = 0.003), middle dose (P = 0.04 (P = 0.02), the percentage of CD3 + T lymphocytes in peripheral blood of oyster peptide-fed group increased compared with that of the model group, while the high dose of oyster peptide in peripheral blood CD8 + T lymphocytes (P = 0.002). The concentration of IL-5 in peripheral blood of mice orally administered oysterin in each dose group was significantly lower than that in the model group (P <0.01), while the high dose The concentration of IL-17 in the peripheral blood of oyster peptide-fed mice was significantly lower than that of the control group (P = 0.03). The concentration of nucleated cells in the bone marrow of mice orally administered with oyster peptide was significantly higher than that of the model group (Low dose group vs. model group, P = 0.04; middle dose group vs model group, P = 0.02; high dose group vs. model group, P = 0.01). Compared with the model group Also significantly increased (P value <0.01). Conclusion: Oyster peptide can improve the structure of immune organs in mice with immunosuppressed mice induced by CTX, restore the imbalance of T lymphocytes and cytokine in mice caused by CTX, increase the number of bone marrow nucleated cells and the content of bone marrow DNA, Immune Function.