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用ＰＣＲ方法特异性扩增ＳＥＲＡ基因片段，并将该基因片段克隆于Ｍ１３噬菌体，用双脱氧链末端终止法进行测序，应用ＰＣＧＥＮＥ软件对该基因序列进行了分析，结果显示该基因片段长度为４５３ｂｐ，Ａ＋Ｔ／Ｇ＋Ｃ为２．６∶１，符合恶性疟原虫结构基因的特点。同源性分析显示该基因在不同虫株间高度保守，我国ＰＦＤ－３／ＹＮ虫株ＳＥＲＡ基因片段仅与Ｂ１、Ｂ２、Ｂ３（巴西）株及Ｓ１６（塞内加尔）株在第６６７位氨基酸有不同，与ＦＣＲ３（冈比亚）、ＦＣＢＲ（哥化比亚）、及Ｓ１４、Ｓ１５（塞内加尔）等其它虫株ＳＥＲＡ基因序列完全一致。抗原表位预测分析显示该多肽Ｎ端和Ｃ端可能有抗原表位存在。
The specific fragment of SERA gene was amplified by PCR. The fragment was cloned into M13 phage and sequenced by dideoxy chain termination. The sequence of the gene was analyzed by PCGENE software. The result showed that the fragment was 453 bp , A + T / G + C 2.6: 1, in line with the characteristics of Plasmodium falciparum structural gene. Homology analysis showed that the gene was highly conserved among different strains. SERA gene fragment of PFD-3 / YN strain in China was only different from the amino acid at position 667 of strains B1, B2, B3 (Brazil) and S16 (Senegal) , Which is completely consistent with the SERA gene sequences of other strains such as FCR3 (Gambia), FCBR (Colombia) and S14, S15 (Senegal). Epitope predictive analysis showed that there may be epitopes on the N-terminal and C-terminal of the polypeptide.