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【目的】探讨吲哚-3-原醇(indol-3-carbinol,I3C)对人低分化鼻咽癌细胞株CNE-2的作用及其机制。【方法】将对数生长期的CNE-2细胞分成空白对照组和I3C组。空白对照组细胞常规培养,I3C组细胞培养体系中加入I3C(50μmol/L)。各组细胞培养48 h后,采用四甲基偶氮唑盐(MTT)法检测细胞增殖;Annexin V-碘化丙啶/异硫氰酸荧光素(PI/FITC)检测细胞凋亡,Real-time PCR检测细胞中胞外信号调节激酶(ERK)和Bax、Bcl-2基因表达;Western blot法检测细胞中ERK、p-ERK、Bax、Bcl-2蛋白表达,Caspase3活性试剂盒检测Caspase3活性。【结果】I3C组与空白对照组比较,CNE-2细胞增殖率显著下降(P<0.05),凋亡率显著升高(P<0.05),Real-time PCR检测显示I3C组与空白对照组比较,ERK m RNA表达无显著性差异,但Bax m RNA表达水平显著增高(P<0.001),Bcl-2 m RNA表达水平显著下降(P<0.05)。Western blot法检测显示,I3C组与空白对照组比较,ERK总蛋白无明显变化(P>0.05),但p-ERK和Bcl-2蛋白表达显著减少(P<0.05),Bax蛋白表达和Caspase3活性显著增加(P<0.05)。【结论】吲哚-3-原醇能通过抑制ERK信号传导通路激活而抑制CNE-2细胞增值和促进CNE-2细胞凋亡。
【Objective】 To investigate the effect of indole-3-carbinol (I3C) on human poorly differentiated nasopharyngeal carcinoma cell line CNE-2 and its mechanism. 【Method】 CNE-2 cells in logarithmic growth phase were divided into blank control group and I3C group. Blank control group cells routine culture, I3C group cell culture system by adding I3C (50μmol / L). The cells were cultured for 48 h and the cell proliferation was detected by MTT assay. The apoptosis of cells was detected by Annexin V-PI / FITC and Real- time PCR was used to detect the expression of ERK, Bax and Bcl-2, Western blot was used to detect the protein expression of ERK, p-ERK, Bax and Bcl-2. Caspase3 activity was detected by Caspase3 activity kit. 【Results】 The proliferation rate of CNE-2 cells in I3C group was significantly lower than that in blank control group (P <0.05), and the apoptosis rate was significantly increased in I3C group (P <0.05). Real-time PCR results showed that I3C group compared with blank control group (P <0.001). The expression of Bcl-2 mRNA was significantly decreased (P <0.05). The results of Western blot showed that there was no significant change in the total protein of ERK between I3C group and blank control group (P> 0.05), but the expression of p-ERK and Bcl-2 protein was significantly decreased (P <0.05); the protein expression of Bax and Caspase3 activity Significantly increased (P <0.05). 【Conclusion】 Indole-3-propene alcohol can inhibit the proliferation of CNE-2 cells and promote the apoptosis of CNE-2 cells by inhibiting the activation of ERK signaling pathway.