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目的：研究延迟整流性Ｋ＋通道在人肝癌细胞增殖中的作用。方法：采用膜片钳技术的全细胞记录方式记录人肝癌细胞在不同浓度ＥＧＦ中延迟整流性Ｋ＋通道的跨膜电流。利用氚标记的胸腺嘧啶核苷（３ＨＴｄＲ）掺入技术观察ＥＧＦ和Ｋ＋通道阻断剂（ＴＥＡ）对人肝癌细胞ＤＮＡ合成的影响。结果：发现表皮生长因子（ＥＧＦ）促进肝癌细胞增殖的同时使肝癌细胞膜离子通道的跨膜Ｋ＋电流增加。Ｋ＋通道阻断剂四乙胺（ＴＥＡ）能显著抑制ＥＧＦ促进ＤＮＡ合成的作用，具有明显的量效关系和时间依赖性，但对细胞的活力无明显影响。结论：人肝癌细胞的增殖活动需要延迟整流性Ｋ＋通道参与。
Objective: To study the role of delayed rectifier K+ channels in the proliferation of human hepatocellular carcinoma cells. Methods: The whole-cell recording method of patch clamp technique was used to record the transmembrane currents of human hepatoma cells that delayed rectifier K+ channels in different concentrations of EGF. The effect of EGF and K+ channel blocker (TEA) on DNA synthesis of human hepatocellular carcinoma cells was observed using tritiated thymidine (3H-TdR) incorporation technique. RESULTS: It was found that epidermal growth factor (EGF) promoted the proliferation of hepatocellular carcinoma cells and increased the transmembrane K+ current of the membrane channel of hepatoma cells. K+ channel blocker tetraethylammonium (TEA) can significantly inhibit the effect of EGF on DNA synthesis. It has a significant dose-effect relationship and time dependence, but it has no obvious effect on cell viability. CONCLUSION: The proliferation of human hepatoma cells requires delayed rectifier K+ channels.