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目的 研究发酵支原体抗HIV 1作用机制。方法 以发酵支原体PG18为实验菌株 ,制备支原体培养上清液和支原体菌体蛋白。用高效液相层析纯化发酵支原体PG18培养上清的蛋白 ,检测各部分逆转录酶抑制活性和核酸酶活性。用ELISA法检测发酵支原体对正常人PBMC及感染HIV 1的人PBMC分泌IL 10的影响及rhIL 10在体外对HIV 1复制的抑制作用。结果 发酵支原体PG18培养上清蛋白质相对分子质量 (Mr)为 (6 7～ 10 0 )× 10 3 和 (10～ 2 5 )× 10 3 时 ,分别具有 36 %和 34 %的RT活性抑制作用和部分核酸酶活性。发酵支原体PG18菌体蛋白可显著诱导正常人PBMC及感染HIV 1的PBMC分泌IL 10 ,且对感染HIV 1的PBMC分泌IL 10的诱导活性更强。rhIL 10可抑制HIV 1在PBMC中的复制 ,并随剂量的增加而增强。结论 发酵支原体PG18培养上清的逆转录酶活性抑制作用和菌体蛋白对PBMC分泌IL 10的促进作用为发酵支原体PG18抗HIV作用的相关机制
Objective To study the mechanism of anti-HIV 1 effect of Mycoplasma fermentum. Methods Fermentation mycoplasma PG18 was used as experimental strain to prepare mycoplasma culture supernatant and mycoplasma bacterial protein. Purified mycoplasma PG18 culture supernatant protein by high performance liquid chromatography to detect each part of the reverse transcriptase inhibitory activity and nuclease activity. The effect of Mycoplasma fermentum on IL-10 secreted by human PBMC and human PBMC infected with HIV-1 and the inhibitory effect of rhIL 10 on HIV-1 replication in vitro were detected by ELISA. Results The results showed that the relative molecular mass of the culture supernatant of the fermented mycoplasma PG18 was (6 7 ~ 10 0) × 10 3 and (10 ~ 2 5) × 10 3, respectively, with 36% and 34% Partial nuclease activity. The mycoplasma fermentative PG18 protein could significantly induce the secretion of IL-10 by PBMCs infected with HIV-1 and PBMC infected by HIV-1, and induced the secretion of IL-10 by PBMCs infected with HIV-1. rhIL 10 inhibited the replication of HIV 1 in PBMCs and increased with increasing doses. Conclusion The inhibitory effect of reverse transcriptase activity of mycoplasma fermentative PG18 and the promotion of IL 10 secreted by PBMC by PBMC are the mechanisms of anti-HIV effect of mycoplasma PG18