论文部分内容阅读
用肺腺癌细胞系Ａ５４９免疫ＢＡＬＢ／Ｃ小鼠，常规细胞融合，获得一株ⅠｇＭ型单抗３Ｃ９。ｗｅｓｔｅｒｎｂｌｏｔ研究发现该抗体识别５６．４ＫＤ蛋白带。免疫组化研究发现，３Ｃ９相关抗原（３Ｃ９Ａｇ）在肺癌组织中表达阳性率高达９２．５％。３Ｃ９Ａｇ血清学研究发现，其诊断肺癌的敏感性为６４．７％，特异性为９３．０％，准确性为８２．５％。其血清水平与组织学分布相关，在高、中分化的肺癌病人阳性率高于低分化者（Ｐ＜０．０５）。表明检测血清３Ｃ９Ａｇ具有重要意义。
The lung adenocarcinoma cell line A549 was used to immunize BALB/C mice and routine cell fusion was performed to obtain a IgM type monoclonal antibody 3C9. Westernblot study found that this antibody recognizes 56.4KD protein band. Immunohistochemical study found that the positive rate of 3C9-associated antigen (3C9Ag) in lung cancer tissues was as high as 92.5%. 3C9Ag serological studies found that its sensitivity for the diagnosis of lung cancer was 64.7%, specificity was 93.0%, and accuracy was 82.5%. The serum levels were correlated with histological distribution. The positive rate of patients with high and moderately differentiated lung cancer was higher than those with poor differentiation (P<0.05). It shows that the detection of serum 3C9Ag is of great significance.