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目的 :探讨当归对兔肾缺血再灌注损伤的防治作用及其机制。方法 :健康成年日本大耳白兔 2 5只 ,随机均分为假手术对照 (control)组、单纯缺血再灌注 (IR)组和缺血再灌注 +当归 (RAS +IR)组。在肾缺血 1h再灌注4 8h后取肾组织作电镜检查 ,并测血清肌酐 (Cr)、肾组织肿瘤坏死因子 (TNF -α)、白细胞介素 - 6 (IL - 6 )和碱性成纤维细胞生长因子 (bFGF)含量。结果 :IR组肾组织变性改变显著 ,RAS +IR组病变轻微 ;IR组Cr、TNF -α和IL - 6含量显著高于control组 (P <0 .0 5 ,P <0 0 5和P <0 0 1) ;RAS +IR组上述指标显著低于IR组。IR组bFGF含量显著低于control组 (P <0 0 1) ,RAS +IR组bFGF含量显著高于IR组 (P <0 0 1)和control组 (P <0 0 5 )。结论 :当归具有防治肾IR损伤的作用 ,其机制可能与其对TNF -α、IL - 6和bFGF等细胞因子的调控有关。
Objective: To investigate the prevention and treatment effect of Angelica sinensis on renal ischemia-reperfusion injury in rabbits and its mechanism. Methods: Twenty-five healthy adult Japanese white rabbits were randomly divided into sham control group, IR group and RAS + IR group. The kidneys were harvested for electron microscopy at 1 h after renal ischemia and 48 h after reperfusion. Serum creatinine (Cr), tumor necrosis factor - α (TNF - α), interleukin - 6 (IL - 6) Fibroblast growth factor (bFGF) content. Results: The degeneration of renal tissue in IR group was significant, and the lesion in RAS + IR group was mild. The levels of Cr, TNF - α and IL - 6 in IR group were significantly higher than those in control group (P <0.05, P <0.05 and P < 0 0 1). The above indexes in RAS + IR group were significantly lower than those in IR group. The content of bFGF in IR group was significantly lower than that in control group (P <0.01). The content of bFGF in RAS + IR group was significantly higher than that of IR group (P <0.01) and control group (P <0 05). Conclusion: Angelica sinensis has the effect of preventing and treating renal IR injury, and its mechanism may be related to the regulation of cytokines such as TNF-α, IL-6 and bFGF.