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目的：探讨Mepyramine对肝脏疾病肝微粒体膜流动性的影响。方法：以1,6diphenyl1,3,5hexatriene(DPH)作为荧光探剂，应用荧光偏振技术研究大鼠急性肝炎、大鼠肝硬化肝微粒体膜流动性（LMMF）的变化，并在体外实验观察mepyramine(MPR)对LMMF的影响。结果：① LMMF在大鼠发生急性肝炎时升高，与对照组相比有显著性差异(P<0.01）,而大鼠发生肝硬化时降低，与对照组相比有显著性差异(P<0.05）。②10-4mol/L MPR可使正常大鼠、大鼠肝硬化及人体肝癌组织LMMF升高(P分别小于0.01，0.01，0.05)。结论：肝病时LMMF出现异常；MPR可以升高LMMF，其机制可能与阻断抗组胺药敏感性细胞色素P450(HP450)有关。
Objective: To investigate the effect of Mepyramine on the fluidity of liver microsomes in liver diseases. Methods: Fluorescence polarization technique was used to study the changes of liver microsomal membrane fluidity (LMMF) in rats with acute hepatitis and liver cirrhosis. The effects of mepyramine (MPR) on LMMF. Results: ① The level of LMMF in acute hepatitis increased significantly compared with that in control group (P <0.01), but decreased in cirrhotic rats (P < 0.05). ②10-4mol / L MPR can increase the LMMF in normal rats, rats with cirrhosis and human liver cancer (P <0.01, 0.01, 0.05 respectively). CONCLUSION: LMMF is abnormal in liver disease. MPR can increase LMMF, which may be related to the block of anti-histamine sensitive cytochrome P450 (HP450).