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目的探讨维生素K2(VK2)联合5-氟尿嘧啶(5-FU)对肝癌细胞体外增殖、迁移和侵袭的影响。方法体外培养人肝癌PLC/RAF/5细胞,分别用VK2(100μmol/L)、5-FU(10μg/m L)及VK2与5-FU联合用药处理细胞24 h,另设置不加药的细胞作为对照组,分别采用CCK-8法、划痕试验、Matrigel侵袭小室法检测细胞增殖、迁移及侵袭情况。结果 VK2或5-FU单独作用细胞后,PLC/RAF/5细胞增殖、迁移和侵袭能力较对照组均明显降低(P<0.05);而与任一单独用药比较,VK2与5-FU联合用药对细胞增殖、迁移和侵袭能力的抑制作用均进一步明显增强(P<0.05)。结论 VK2与5-FU联合应用可能成为抑制肝癌细胞生长、迁移和侵袭的有效方法。
Objective To investigate the effects of vitamin K2 (VK2) combined with 5-fluorouracil (5-FU) on the proliferation, migration and invasion of hepatocellular carcinoma cells in vitro. Methods Human hepatocarcinoma PLC / RAF / 5 cells were cultured in vitro and treated with VK2 (100μmol / L), 5-FU (10μg / mL) and VK2 combined with 5-FU for 24 hours respectively. As a control group, cell proliferation, migration and invasion were detected by CCK-8 assay, scratch assay and Matrigel invasion chamber method. Results Compared with control group, the proliferation, migration and invasion ability of PLC / RAF / 5 cells were significantly decreased after VK2 or 5-FU treatment (P <0.05). However, VK2 combined with 5-FU The inhibitory effect on cell proliferation, migration and invasion were further enhanced (P <0.05). Conclusion VK2 combined with 5-FU may be an effective method to inhibit the growth, migration and invasion of hepatocellular carcinoma cells.