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A sensitive reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay was developed for rapid visual detection of Murray valley encephalitis virus(MVEV)infection.The reaction was performed in one step in a single tube at 63°C for 60 min with the addition of the hydroxynaphthol blue(HNB)dye prior to amplification.The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 ° C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold