论文部分内容阅读
目的:研究补肾健脾法与清热燥湿法比较对高脂喂养2型糖尿病小鼠肝脏组织炎症因子水平的影响。方法:用高脂饲料诱导2型糖尿病小鼠模型,分别予以补肾健脾法及清热燥湿法干预。测定各组小鼠血糖,IL-1β、TNF-αmRNA水平,HE染色观察小鼠肝脏组织结构变化。结果:(1)与普食组小鼠对比高脂组小鼠血糖水平明显升高(P<0.05),清热燥湿组、早期补肾健脾组(早脾肾组)小鼠血糖较高脂组小鼠有明显的降低(P<0.05),晚期补肾健脾组(晚脾肾组)血糖值较高脂组无显著差异;(2)高脂组小鼠与普食组比较,肝脏组织中炎症因子IL-1β、TNF-αmRNA水平也明显升高(P<0.05);清热燥湿组、早脾肾组、晚脾肾组炎症因子IL-1β、TNF-αmRNA水平较高脂组有明显的下降(P<0.05);(3)组织切片HE染色可以看出高脂组小鼠肝脏细胞出现十分明显的脂滴增多,早脾肾组、清热燥湿组小鼠HE染色可以看到肝脏细胞内脂滴明显减少,晚脾肾组较高脂组肝内脂质沉积也有一定缓解。结论:早期补肾健脾法干预可以降低2型糖尿病小鼠血糖,并降低小鼠肝脏炎症因子mRNA水平,保护肝脏。
Objective: To study the effects of tonifying kidney and invigorating the spleen method and heat-clearing and damp-drying method on the levels of inflammatory cytokines in the liver tissue of type 2 diabetic mice fed with high fat diet. Methods: Type 2 diabetes mellitus mice were induced with high-fat diet and were given the method of tonifying kidney and invigorating the spleen and clearing heat and dampness respectively. The blood glucose, IL-1β and TNF-αmRNA levels of the mice in each group were measured, and the changes of hepatic tissue structure were observed by HE staining. Results: (1) Compared with the mice in the normal control group, the blood glucose levels of the mice in the high-fat diet group were significantly increased (P <0.05). The blood glucose of the mice in the heat-dampness dampness group, the early Bushenjianpi group (P <0.05). There was no significant difference in blood glucose level between the late Bushen Jianpi group (late spleen and kidney group) and the high fat group. (2) Compared with the control group, the liver tissue IL-1β and TNF-αmRNA in the heat-dampness group, the early spleen-kidney group and the late-spleen-kidney group were significantly higher than those in the high-fat group (P <0.05) (P <0.05). (3) HE staining showed that there were obvious lipid droplets in the liver cells of the high-fat diet group. HE staining showed that the mice in the early spleen and kidney group and the Qingre dampness group could be seen by HE staining Lipid droplets in liver cells were significantly reduced, the late spleen and kidney group compared with high-fat group also had some relief of intrahepatic lipid deposition. Conclusion: The early intervention of invigorating the kidney and invigorating the spleen can decrease the blood sugar of mice with type 2 diabetes mellitus and reduce the mRNA level of liver inflammation in mice and protect the liver.