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目的研究人浆细胞瘤转化迁移基因(PVT1)rs2720709的A/G多态性与糖尿病(DM)肾病(DN)易感性的关系。方法将连云港市225例2型糖尿病患者分为DN组[微量白蛋白/肌酐比值(ACR)>30mg/g,135例]和DM组(ACR≤30mg/g,90例);另选94例健康人群为正常对照(NC组)。抽取外周静脉血,提取基因组DNA,PCR技术扩增目标基因并测序鉴定,比较各组等位基因及基因型频率。结果三组PVT1基因的分布符合Hardy-Weinberg定律,基因型频率和等位基因频率差异均无统计学意义(P>0.05)。结论 PVT1基因rs2720709的A/G多态性与该地区汉族人的DN易感性无关。
Objective To study the relationship between A / G polymorphism of human plasmacytoma transforming tumor (PVT1) rs2720709 and the susceptibility to diabetic nephropathy (DN). Methods One hundred and twenty-five patients with type 2 diabetes in Lianyungang were divided into DN group (ACR> 30mg / g, 135 cases) and DM group (ACR≤30mg / g, 90 cases) Healthy people as a normal control (NC group). Peripheral venous blood was extracted and genomic DNA was extracted. The target gene was amplified by PCR and sequenced. The alleles and genotype frequencies of each group were compared. Results The distribution of PVT1 gene in three groups accorded with Hardy-Weinberg law, and there was no significant difference in genotype frequency and allele frequency (P> 0.05). Conclusion The A / G polymorphism of rs2720709 in PVT1 gene is not associated with the susceptibility to DN in Han population in this area.