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目的探讨生长抑素和5-FU联合应用对结肠癌裸鼠原位移植瘤生长的抑制作用和机制。方法建立结肠癌裸鼠原位移植瘤模型,分为4组,每组12只。分为善宁组,腹腔注射善宁(100μg·kg-1.d-1);善宁+5-FU组,注射善宁(100μg·kg-1.d-1)+5-FU(30mg·kg-1.d-1);5-FU组,注射5-FU(30mg·kg-1.d-1);对照组,注射生理盐水。结果善宁组、善宁+5-FU组、5-FU组、对照组抑瘤率分别为42.0%、86.2%、79.7%、0(P<0.05);MVD分别为2.3±1.4、0.9±0.5、12.1±4.3、13.5±5.1(P<0.05);AI分别为11.6±3.8、19.7±8.5、7.0±5.6、3.5±2.3(P<0.05);腹膜转移率分别为20.0%、0、50.0%、100%(P<0.05);肝转移率分别为20.0%、0、40.0%、85.7%(P<0.05);腹水发生率分别为10.0%、0、20.0%、28.6%(P<0.05);肺转移率分别为10.0%、0、20.0%、42.9%(P<0.05)。按照抑瘤由强到弱的效果排列顺序为善宁+5-FU、5-FU、善宁。结论联合应用生长抑素和5-FU,比单独应用传统化疗药物能显著抑制结肠癌的生长和转移。
Objective To investigate the inhibitory effect of somatostatin and 5-FU on the growth of xenografted xenografts in nude mice and its mechanism. Methods The orthotopic colon xenograft model of colon cancer was established and divided into 4 groups with 12 in each group. The rats were divided into Shanyin group, intraperitoneal injection of Shannon (100μg · kg -1 · d -1), Shannon + 5-FU group, injection of 100μg · kg -1 · d -1 + 5-FU · Kg-1.d-1); 5-FU group, injected with 5-FU (30mg · kg-1.d-1); Results The tumor inhibition rate of Shanning group, Shanning + 5-FU group, 5-FU group and control group were 42.0%, 86.2%, 79.7%, 0 respectively (P <0.05); MVD was 2.3 ± 1.4,0.9 ± AI were 11.6 ± 3.8, 19.7 ± 8.5, 7.0 ± 5.6 and 3.5 ± 2.3, respectively (P <0.05). The peritoneal metastasis rates were 20.0%, 0,50.0 (P <0.05). The rates of hepatic metastasis were 20.0%, 0,40.0% and 85.7% (P <0.05) respectively. The incidence of ascites was 10.0%, 0,20.0% and 28.6% ). The lung metastasis rates were 10.0%, 0,20.0% and 42.9%, respectively (P <0.05). In accordance with the anti-tumor effect from strong to weak order of good Ning + 5-FU, 5-FU, Shannon. Conclusions Combination of somatostatin and 5-FU can significantly inhibit the growth and metastasis of colon cancer than the traditional chemotherapy alone.