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本文用火箭电泳法和酶联免疫吸附法分别测定了人奶和不同喂养方式新生儿粪便乳铁蛋白(LF)。结果显示,正常产妇1～8天初乳LF含量为6.88±3.10mg/ml,1～4个月成熟乳为3.18±0.87mg/ml。产后头3天初乳LF含量最高,之后渐下降,成熟乳阶段趋于稳定。不同喂养方式新生儿粪便LF含量有非常显著性差异(P<0.01)。粪便LF检出率,母乳喂养儿为100％,人工喂养儿仅60％。胎便中可检出小量,检出率为35％。母乳与新生儿粪便LF同步测定结果显示,母乳中高浓度LF对母乳喂养儿肠道LF含量有肯定的影响。人体肠道内源性LF的产生,从胎儿期就已开始,但在新生儿早期产生十分有限。母乳喂养能通过向新生儿传递包括LF在内的多种免疫物质,弥补生命早期自体产生的不足。
In this paper, rodent electrophoresis and enzyme-linked immunosorbent assay were used to determine fecal lactoferrin (LF) in human milk and different feeding neonates. The results showed that the normal pregnant women 1 to 8 days of colostrum LF content of 6.88 ± 3.10mg / ml, 1 to 4 months of mature milk was 3.18 ± 0.87mg / ml. The first 3 days postpartum colostrum LF content highest, then gradually decreased, mature milk stage tends to be stable. There was a significant difference in LF content of newborn feces among different feeding ways (P <0.01). LF detection rate of faeces, breastfeeding children to 100%, only 60% of artificially fed children. A small amount can be detected in the stool, the detection rate was 35%. Simultaneous determination of LF in feces and neonates showed that the high concentration of LF in breast milk had a positive effect on the LF content in the intestine of breastfeeding children. The production of endogenous LF in the human gut has started from the fetal stage but is very limited in the early neonates. Breastfeeding can compensate for the self-generated deficits early in life by delivering a variety of immune substances, including LF, to newborns.