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田间试验条件下,以转双价(Bt+CpTI)基因棉SGK321及其非转基因亲本常规棉石远321为研究对象,比较分析不同生育期(30、60、90、120d)转双价基因棉和亲本棉根际土壤酶活性(脲酶、碱性磷酸酶、过氧化氢酶)及速效养分(硝态氮、铵态氮和速效磷)的变化。结果表明,转双价基因棉根际土壤硝态氮、铵态氮和速效磷含量变化趋势与其亲本常规棉基本一致,但各养分的具体变化幅度因生育期不同而异。播种后30、60、120d转双价基因棉和亲本常规棉根际土壤碱性磷酸酶活性无显著差异;土壤脲酶和过氧化氢酶活性随棉花生育进程其变化趋势虽各不相同,但同一生育期转双价基因棉与亲本常规棉根际土壤脲酶和过氧化氢酶活性均无显著差异。表明,土壤酶活性和速效养分含量受转双价基因棉的影响较小,其变化主要受生育期的影响。
Under the field experiment, transgenic Bt + CpTI cotton SGK321 and its non-transgenic parental conventional cotton stretcher 321 were used as research objects to compare the effects of different transgenic cotton (Bt + CpTI) transgenic cotton (Bt + CpTI) And the changes of soil enzyme activities (urease, alkaline phosphatase and catalase) and available nutrients (nitrate nitrogen, ammonium nitrogen and available phosphorus) in the rhizosphere soil of the parents and their parents. The results showed that the contents of nitrate nitrogen, ammonium nitrogen and available phosphorus in rhizosphere soil of transgenic Bt cotton were basically the same as that of their conventional cotton, but the variation range of each nutrient varied with the growth period. There was no significant difference in alkaline phosphatase activity between rhizosphere soil and transgenic cotton at 30, 60 and 120 days after sowing. The trend of soil urease and catalase activity varied with the growth of cotton, but the same There was no significant difference in urease and catalase activities between rhizosphere cotton and transgenic cotton during the growth period. The results showed that soil enzyme activity and available nutrient content were less affected by transgenic cotton, which was mainly affected by the growth period.